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. Author manuscript; available in PMC: 2021 Feb 19.
Published in final edited form as: ACS Chem Neurosci. 2020 Jul 9;11(15):2316–2326. doi: 10.1021/acschemneuro.0c00276

Figure 2.

Figure 2.

Synthesis and fluorescence of a far-red environment sensor. (A) Synthesis of JP-N3 by condensation of nitroso julolidine and hydroxymethyl resorcinol. DPPA: Diphenyl phosphorazidate; DBU: 1,8-diazabicyclo[5.4.0]undec-7-ene. (B) JP environment sensitivity. Excitation (dashed) and emission (solid lines) spectra of JP-N3 in nonpolar and polar solvents. (C) Enhanced brightness and stability (inset) conferred by julolidine rings. Emission measured by epifluorescence microscopy with 550-nm excitation of AP (ref31) or JP adducts to Lys27Pra on Kv2.1-expressing CHO cells.