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. Author manuscript; available in PMC: 2021 Feb 19.
Published in final edited form as: ACS Chem Neurosci. 2020 Jul 9;11(15):2316–2326. doi: 10.1021/acschemneuro.0c00276

Figure 4.

Figure 4.

GxTX-JP conjugate fluorescence from cell membranes depends on Kv2.1 channels and membrane potential. Normalized fluorescence of Lys27Pra(JP) GxTX on CHO cells (A) expressing Kv2.1 channels at −80 mV, (B) expressing Kv2.1 channels at +40 mV, (c) without Kv2 channels at −80 mV, and (b) without Kv2 channels at +40 mV. All cells were excited at 543 nm, and component peaks shorter than 600 nm in (C) and (D) likely represent cellular autofluorescence rather than JP emission. Data points are mean ± SEM of normalized emission from 4 cells. All spectra are shown with 2-component split pseudo-Voigt fits (black) with individual components (blue, red). Fit parameters and R2 values are listed Table S1.