FIGURE 7.

Osteopontin is responsible of the oligodendrocyte precursor cell (OPC) survival due to myeloid‐derived suppressor cell (MDSC) secretome. (a–c) Isolated OPCs in culture in the different experimental conditions, immunostained with Olig2 (red fluorescence), and TUNEL to detect apoptotic cells (green fluorescence). (d) The better survival observed in MDSC conditioned medium (MDSC‐CM) came back to control levels when osteopontin was eliminated from that (MDSC‐CM‐OPN condition). Arrows indicate double‐labeled cells. Scale bar represents 53 μm in (a–c). Results of one‐way analysis of variance (ANOVA) with post hoc Tukey test (n = 4) are represented as: *p < .05