FIGURE 8.

Osteopontin is the main effector of myeloid‐derived suppressor cell (MDSC) conditioned medium in promoting oligodendrocyte precursor cell (OPC) proliferation. (a–c) Images of A2B5⁺ cells in culture in different conditions, immunostained with Olig2 (red fluorescence) and showing BrdU (green fluorescence) incorporation. The increase of OPC proliferation observed with MDSC conditioned medium (MDSC‐CM) came back to control levels when osteopontin was eliminated from that (MDSC‐CM‐OPN condition; d). Arrows indicate double‐labeled cells. Scale bar represents 53 μm in (a–c). Results of multicomparison with post hoc Tukey test (n = 4) are represented as: *p < .05 and **p < .01