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. 2021 Feb 5;12:630561. doi: 10.3389/fneur.2021.630561

Figure 6.

Figure 6

Validating expression of genes implicated in Meniere's disease in the adult mouse SV. (A,A′ ) RNA probes demonstrate co-expression of Esrrb (red) and Atp1b2 (green) RNA in marginal cells of the adult mouse SV (P30 mouse) with DAPI (white) labeling of cell nuclei (A) and without DAPI labeling of cell nuclei (A′ ). Scale bars are 20 microns. (B,B′ ) RNA probes demonstrate co-expression of Esrrb (red) and Kcne1 (green) RNA in marginal cells of the adult mouse SV (P90 mouse) with DAPI (white) labeling of cell nuclei (B) and without DAPI labeling of cell nuclei (B′ ). Scale bars are 20 microns. (C,C′ ) RNA probes demonstrate co-expression of Met (turquoise), Ednrb (red) and Tmem176a (green) RNA in the intermediate cell layer of the adult mouse SV (P90 mouse) with DAPI labeling of cell nuclei (C) and without DAPI labeling of cell nuclei (C′ ). Scale bars are 20 microns. Yellow dashed line boxes outline representative cells where the RNA signal of all 3 probes are colocalized to intermediate cell nuclei. (D,E) Closeup images of representative cells #1 (D) and #2 (E) from (C,C′ ) where RNA expression of Met (turquoise), Ednrb (red), and Tmem176a (green) are co-expressed in intermediate cell nuclei. Scalebars are 10 microns.