Table 2.

Demonstrates examples of contingencies in place and their grading in case of operator error or equipment failures throughout pipeline.

Issue	Contingency	Grade
Aspirator breaks.	Use multi‐channel pipette to remove wash.	Green
No precoated plates prepared.	Masterplate stored in fridge at 4°C for up to 72 h.	Green
Barcode scanner breaks.	Manually record the barcode number in the paper log and on the 2 ml tube, proceed as normal.	Amber
Clumpy/hemolyzed sample upon aliquoting.	Record this information in log and continue.	Amber
Plate reader failure.	Add 50 μl of 1 M NaOH to quench plate at 15 min after addition of substrate, store in dark by wrapping in foil at room temperature until problem can be resolved.	Red
	If >12 h repeat assay.
Any steps not completed as per SOP/missed.	Terminate assay and repeat next day.	Red