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. 2021 Feb 19;129(2):027005. doi: 10.1289/EHP7297

Figure 8.

Figures 8A, 8B, 8C, and 8D are a set of images of M D A-M D-231 cell proliferation, Figure 8E is a bar graph. In Figures 8A, 8B, 8C, and 8D, the cell proliferation effects of bisphenol S on M D A-M D-231 cells with 200 micrometers are presented. M D A-M D-231 cells were exposed to 5-ethynyl-2′-deoxyuridine 10 micromolar for 3 hours, vehicle at 0.1 percent Dimethyl sulfoxide, epidermal growth factor at 10 nanograms per milliliter, or bisphenol S at 200 nanograms per milliliter. Nuclei were stained with 4′,6-diamidino-2-phenylindole. The cells were exposed for 72 hours. In Figure 8E, the bar graph titled M D A-M D-231 plots percentage of ethynyl-2′-deoxyuridine positive cells, ranging from 90 to 110 in increments of 5 (y-axis) across vehicle, bisphenol S, epidermal growth factor, and bisphenol S plus epidermal growth factor (x-axis).

Effect of BPS exposure on the proliferation of MDA-MD-231 cells. (A–D) Representative images of MDA-MD-231 cells exposed to 5-ethynyl-2′-deoxyuridine (EdU; 10μM) for 3 h (EdU-positive, green). Nuclei were stained with DAPI (blue) following 72-h exposure to vehicle (0.1% DMSO) (A); BPS (200 ng/mL) (B); EGF (10 ng/mL) (C); or BPS+EGF (D) (same doses as single groups). (E) Cell proliferation (mean±SEM) was expressed as the percentage of EdU-positive cells of the total number of nuclei and normalized to the vehicle group. n=3/group. A generalized linear model was used to compare treatments. Different letters denote statistical differences among treatment groups at p<0.05. Note: BPS, bisphenol S; DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide; EGF, epidermal growth factor; SEM, standard error of the mean.