Figure 4.
Effects of inhibition of hsa_circ_0071036 on pancreatic cancer cell proliferation, colony formation, invasion and apoptosis. (a) Characterization of the KRAS gene and hsa_circ_0071036 expression in the human PDAC cell lines PANC-1, AsPC-1 and BxPC-3. Left, DNA sequencing confirmed the presence of wild-type KRAS in BxPC-3 cells and mutant KRASG12D in PANC-1 and AsPC-1 cells. Right, qRT-PCR analyses of hsa_circ_0071036 expression in various PDAC cells. (b) CCK-8 analysis revealed that inhibition of hsa_circ_0071036 suppressed the proliferation of PANC-1 cells (one-way ANOVA). (c) Invasion assays showed that knockdown of hsa_circ_0071036 attenuated the invasion ability of PANC-1 cells (Student’s t test, mean ± SEM). (d) Flow cytometry revealed that knockdown of hsa_circ_0071036 induced late apoptosis in PANC-1 cells (Student’s t test, mean ± SEM). (e) Colony formation assay showed that hsa_circ_0071036 expression had the same effect as obtained by the CCK-8 assay on PANC-1 cell growth ability (Student’s t test, mean ± SEM)