Figure 5.

Sox4 attenuates MyD88-dependent and independent pathways and represses MyD88 expression. (a and b) L02 cells were transfected with pFlag-Sox4 and infected with SeV. Proteins expressed in the cells were determined by Western blot analyses (a). Whole cell extracts were prepared for Co-IP using antibody to MyD88, and the precipitates were analyzed using antibodies to IRAK4, IRAK1, or MyD88 (B, top). MyD88 and GAPDH proteins in the whole cell lysates (WCLs) were analyzed by Western blots (B, bottom). (c–e) L02 cells (c), HepG2 cells (d), and PBMCs (e) were transfected with pFlag-Sox4 or pFlag2A. MyD88 mRNAs expressed in the cells was determined by RT-PCR. The results are shown as means ± SD (n = 3). **P < 0.05. (f–k) L02 cells (f), HepG2 cells (g), and PBMCs (h) were transfected with pFlag-Sox4 or pFlag2A. Huh7 cells were transfected with siR-Sox4 or siR-NC (i). L02 cells were transfected with pFlag-Sox4, pFlag-Sox4ΔHMG, or pFlag-Sox4ΔTAD (j). L02 cells were transfected with pFlag-Sox4 or pFlag2A and then treated with CHX (k). MyD88, Sox4, GFP, and GAPDH proteins expressed in the cells were detected by Western blot analyses