Table 1.
Primers used in this study
| Name of the primer | Sequence of the primer |
|---|---|
| Sox4-rqF | 5ʹ-GTGAGCGAGATGATCTCGGG-3’ |
| Sox4-rqR | 5ʹ-CAGGTTGGAGATGCTGGACTC-3’ |
| IFN-α-rqF | 5ʹ-TTTCTCCTGCCTGAAGGACAG-3’ |
| IFN-α-rqR | 5ʹ-GCTCATGATTTCTGCTCTGACA-3’ |
| IFN-β-rqF | 5ʹ-TGGGAGGCTTGAATACTGCCTCAA-3’ |
| IFN-β-rqR | 5ʹ-TCCTTGGCCTTCAGGTAATGCAGA-3’ |
| IFN-λ1 F-rqF | 5ʹ-CTTCCAAGCCCACCACAACT-3’ |
| IFN-λ1 R-rqR | 5ʹ-GGCCTCCAGGACCTTCAGC-3’ |
| TLR1-rqF | 5ʹ-CAGTGTCTGGTACACGCATGGT-3’ |
| TLR1-rqR | 5ʹ-TTTCAAAAACCGTGTCTGTTAGAGA-3’ |
| TLR2-rqF | 5ʹ-GGCCAGCAAATTACCTGTGTG-3’ |
| TLR2-rqR | 5ʹ-AGGCGGACATCCTGAACCT-3’ |
| TLR3-rqF | 5ʹ-CCTGGTTTGTTAATTGGATTAACGA-3’ |
| TLR3-rqR | 5ʹ-TGAGGTGGAGTGTTGCAAAGG-3’ |
| TLR4-rqF | 5ʹ-CAGAGTTTCCTGCAATGGATCA-3’ |
| TLR4-rqR | 5ʹ-GCTTATCTGAAGGTGTTGCACAT-3’ |
| TLR5-rqF | 5ʹ-TGCCTTGAAGCCTTCAGTTATG-3’ |
| TLR5-rqR | 5ʹ-CCAACCACCACCATGATGAG-3’ |
| TLR6-rqF | 5ʹ-GAAGAAGAACAACCCTTTAGGATAGC-3’ |
| TLR6-rqR | 5ʹ-AGGCCAAACAAAATGGAAGCTT-3’ |
| TLR7-rqF | 5ʹ-TTTACCTGGATGGAAACCAGCTA-3’ |
| TLR7-rqR | 5ʹ-TCAAGGCCTGAGAAGCTGTAAGCTA-3’ |
| TLR8-rqF | 5ʹ-TTATGTGTTCCAGGAACTCAGAGAA-3’ |
| TLR8-rqR | 5ʹ-TAATACCCAAGTTGATAGTCGATAAGTTTG-3’ |
| TLR9-rqF | 5ʹ-GGACCTCTGGTACTGCTTCCA-3’ |
| TLR9-rqR | 5ʹ-AAGCTCGTTGTACACCCAGTCT-3’ |
| TLR10-rqF | 5ʹ-TGTTATGACAGCAGAGGGTGATG-3’ |
| TLR10-rqR | 5ʹ-GAGTTGAAAAAGGAGGTTATAGGATAAATC-3’ |
| p65-rqF | 5ʹ-CCTTCCAAGAAGAGCAGCGT-3’ |
| p65-rqR | 5ʹ-GATCTTGAGCTCGGCAGTGT-3’ |
| p50-rqF | 5ʹ-CCAACAGATGGCCCATACCT-3’ |
| p50-rqR | 5ʹ-AACCTTTGCTGGTCCCACAT-3’ |
| IRF3-rqF | 5ʹ-ACCAGCCGTGGACCAAGAG-3’ |
| IRF3-rqR | 5ʹ-TACCAAGGCCCTGAGGCAC-3’ |
| IRF7-rqF | 5ʹ-TGGTCCTGGTGAAGCTGGAA-3’ |
| IRF7-rqR | 5ʹ-GATGTCGTCATAGAGGCTGTTGG-3’ |
| IAV-NP-vRNA-RT | 5ʹ-CTCACCGAGTGACATCAACATCATG-3’ |
| IAV-NP-cRNA-RT | 5ʹ-AGTAGAAACAAGGGTATTTTTCTTTAATTGTCAT-3’ |
| IAV-NP-rqF | 5ʹ-ATCAGACCGAACGAGAATCCAGC-3’ |
| IAV-NP-rqR | 5ʹ-GGAGGCCCTCTGTTGATTAGTGT-3’ |
| EV71-VP1-rqF | 5ʹ-AATTGAGTTCCATAGGTG-3’ |
| EV71-VP1-rqR | 5ʹ-CTGTGCGAATTAAGGACAG-3’ |
| GAPDH-rqF | 5ʹ-GGAAGGTGAAGGTCGGAGTCAACGG-3’ |
| GAPDH-rqR | 5ʹ-CTCGCTCCTGGAAGATGGTGATGGG-3’ |
The genes detected by real-time PCR are listed. The sequences of each primer are presented. Sox4-rqF, real-time PCR forward primer for Sox4 gene mRNA; Sox4-rqR, real-time PCR reverse primer for Sox4 gene mRNA; IFN-α-rqF, real-time PCR forward primer for IFN-α gene mRNA; IFN-α-rqR, real-time PCR reverse primer for IFN-α gene mRNA; IFN-β-rqF, real-time PCR forward primer for IFN-α gene mRNA; IFN-β-rqR, real-time PCR reverse primer for IFN-α gene mRNA; IFN-λ1-rqF, real-time PCR reverse primer for IFN-λ1 gene mRNA; IFN-λ1-rqR, real-time PCR reverse primer for IFN-λ1 gene mRNA; TLR1-rqF, real-time PCR forward primer for TLR1 gene mRNA; TLR1-rqR, real-time PCR reverse primer for TLR1 gene mRNA; TLR2-rqF, real-time PCR forward primer for TLR2 gene mRNA; TLR2-rqR, real-time PCR reverse primer for TLR2 gene mRNA; TLR3-rqF, real-time PCR forward primer for TLR3 gene mRNA; TLR3-rqR, real-time PCR reverse primer for TLR3 gene mRNA; TLR4-rqF, real-time PCR forward primer for TLR4 gene mRNA; TLR4-rqR, real-time PCR reverse primer for TLR4 gene mRNA; TLR5-rqF, real-time PCR forward primer for TLR5 gene mRNA; TLR5-rqR, real-time PCR reverse primer for TLR5 gene mRNA; TLR6-rqF, real-time PCR forward primer for TLR6 gene mRNA; TLR6-rqR, real-time PCR reverse primer for TLR6 gene mRNA; TLR7-rqF, real-time PCR forward primer for TLR7 gene mRNA; TLR7-rqR, real-time PCR reverse primer for TLR7 gene mRNA; TLR8-rqF, real-time PCR forward primer for TLR8 gene mRNA; TLR8-rqR, real-time PCR reverse primer for TLR8 gene mRNA; TLR9-rqF, real-time PCR forward primer for TLR9 gene mRNA; TLR9-rqR, real-time PCR reverse primer for TLR9 gene mRNA; TLR10-rqF, real-time PCR forward primer for TLR10 gene mRNA; TLR10-rqR, real-time PCR reverse primer for TLR10 gene mRNA; p65-rqF, real-time PCR forward primer for p65 gene mRNA; p65-rqR, real-time PCR reverse primer for p65 gene mRNA; p50-rqF, real-time PCR forward primer for p50 gene mRNA; p50-rqR, real-time PCR reverse primer for p50 gene mRNA; IRF3-rqF, real-time PCR forward primer for IRF3 gene mRNA; IRF3-rqR, real-time PCR reverse primer for IRF3 gene mRNA; IRF7-rqF, real-time PCR forward primer for IRF7 gene mRNA; IRF7-rqR, real-time PCR reverse primer for IRF7 gene mRNA; IAV-NP-vRNA-reverse transcription, reverse transcription primer for IAV NP gene vRNA; IAV-NP-cRNA-reverse transcription, reverse transcription primer for IAV NP gene cRNA; IAV-NP-rqF, real-time PCR forward primer for IAV NP gene mRNA; IAV-NP-rqR, real-time PCR reverse primer for IAV NP gene mRNA; EV71-VP1-rqF, real-time PCR forward primer for EV71 VP1 gene mRNA; EV71-VP1-rqR, real-time PCR reverse primer for EV71 VP1 gene mRNA; GAPDH-rqF, real-time PCR forward primer for GAPDH gene mRNA; GAPDH-rqR real-time PCR reverse primer for GAPDH gene mRNA.