Table 2.

Primers of real-time PCR used in this study for genes detections

Gene	Location of the Sox4 binding site	Sequence of the Sox4 binding site on corresponding TLR promoters
TLR1	−376 p to −370 bp	5ʹ-GGCAAGAGGAAAAACAAAGCAGCCGAAACA-3’
TLR2	none	none
TLR3	+197 bp to +205 bp	5ʹ-TCTTTGGTCTTTCTTTGATCTGGTGCTTGAAAT-3’
TLR4	−997 bp to −990 bp -124 bp to −118 bp	5ʹ-AGGAAGGAGGCTTTGATCTATACTACACAG-3ʹ 5ʹ-CCACAGCTGAACAAAATGGAAAATCAC-3’
TLR5	−1792 bp to −1786 bp -865 bp to −859 bp	5ʹ-AGAATATAAACTTTGTTTGTAGTTCATAGG-3ʹ 5ʹ-TAAGTGCCAGGCTTTGTTTTACACCTATCT-3’
TLR6	−1363 bp to −1357 bp -677 bp to −670 bp	5ʹ-TACTGTCTGTTTCTTTGTTTGCTTAACTGT-3ʹ 5ʹ-TCTGGTAATCAGCCTTTGTTGATGTCATTCT-3’
TLR7	−1937 bp to −1930 bp -78 bp to −71 bp	5ʹ-GTAATGCACCCTTTGTTATATGAAAGGAG-3ʹ 5ʹ-CCGACCTGATCTTTGTAGTTGGAAACT-3’
TLR8	−406 bp to −400 bp	5ʹ-CAGAAACTTGTGGAACAAAGATGAAGCA-3’
TLR9	−761 bp to −754 bp	5ʹ-AAAAAATGTTAGGACAAAGAGAAACATAGA-3’
TLR10	−213 bp to −207 bp +83 bp to +89 bp	5ʹ-CCAGCCTGGGTGACAAAGTGAGACCCTACC-3ʹ 5ʹ-GTAAGAACCTTAGCTTTGTTTGTTGTAACT-3’

Sequence analyses revealed that the promoters of all TLRs genes (excluding TLR2 gene) contain one or two potential Sox4 binding sites. The locations of Sox4 binding sites are indicated. The sequences of Sox4 binding sites on corresponding TLR promoters are presented. The core sequences of the Sox4 binding sites are underlined.