Fig. 5.

Loss of histone H1 and DNA hypomethylation increase Arabidopsis heat tolerance. COPIA78/ONSEN (a) and GYPSY (c) transcripts in wild‐type (WT), h1, WT plus 40 µm zebularine (WT + Z) and h1 plus 40 µm zebularine (h1 + Z). COPIA78/ONSEN (b) and GYPSY (d) transcripts in wt plus heat (WT + heat), h1 plus heat (h1 + heat), WT plus heat and 40 µm zebularine (WT + heat + Z) and h1 plus heat and 40 µm zebularine (h1 +  heat + Z). Error bars represent 1 SD. **, P < 0.01; ns, not significant (Student’s t‐test). Survival rate (e) of seedlings of WT, h1 mutants, WT plus 10 µm zebularine and h1 plus 10 µm zebularine after 36 h heat stress and 48 h recovery under long day conditions. Each of three biological replicates corresponds to ≥ 90 seedlings. The lower and upper hinges of the boxplots correspond to the first and third quartiles of the data, the black lines within the boxes mark the median. Representative pictures (f) of plates with WT, h1 mutants, WT plus 10 µm zebularine and h1 plus 10 µm zebularine after 36 h heat stress and 48 h recovery under long day conditions. Bars, 1 cm.