Fig. 7.
Native ex vivo rates of superoxide/hydrogen peroxide production from isolated mitochondria from 12‐month‐old mice. Mitochondrial H2O2production rate was measured in complex media mimicking the cytosol of (A) heart and (B) skeletal muscle ‘at rest’ (see methods for media composition). Values are mean ± SEM, n ≧ 3 independent mitochondrial preparations and were normalized by mitochondrial protein (ptn). P > 0.05 Student's t‐test. (C) protein levels of MnSOD (SOD2), catalase, PRDX2, PRDX3, and VDAC in whole tissue homogenates from heart and skeletal muscle from wild‐type and tazkd mice. Immunoblots were quantified by densitometry normalized to VDAC levels. mRNA levels of catalase and SOD2 in the heart (D‐E) and skeletal muscle (F‐G). (H) schematic representation of the PRDX2 and PRDX3 catalytic cycle. (I) PRDX3 and PRDX2 dimer/monomer assessed under non‐reducing conditions in the presence of NEM. Immunoblots were quantified by densitometry. Values are mean ± SEM, n ≧ 3.