Table 1.
The overlap proteins that found both with phosphorylation and ubiquitination in buffalo epididymal sperm
| Protein accession | Protein description | Gene name | Phosphorylation sites | Ubiquitination sites |
|---|---|---|---|---|
| Q0IID8 | 1‐Acylglycerol‐3‐phosphate O‐acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) | AGPAT5 | S186 | K209, K120 |
| Q58D01 | 26S proteasome non‐ATPase regulatory subunit 4 | PSMD4 | S266 | K122 |
| P62194 | 26S proteasome regulatory subunit 8 | PSMC5 | S395 | K222 |
| F1MQJ7 | 3‐Phosphoinositide dependent protein kinase 1 | PDPK1 | S242 | K258 |
| A6QLG5 | 40S ribosomal protein S9 | RPS9 | S153, S23 | K30 |
| F1MJS8 | A‐kinase anchor protein 3 | AKAP3 | S246, S821, S485, S157, S52, S210, S33, S177, S103, S36, S179, S183, S205, S611, S765, S61, S75, S431, S215, T212 | K71, K250, K41, K220, K180, K60, K691, K433, K616 |
| F1MYH5 | A‐kinase anchoring protein 4 | AKAP4 | S434, S121, S194, S246, S333, S123, S618, S125, S153, S647, S811, S588, S217, S423, S527, S483, S198, S583, S21, S488 | K74, K486, K804, K502, K523, K590, K645, K614, K808, K367, K821, K31 |
| F1MQJ0 | Angiotensin‐converting enzyme | ACE | S1299 | K832, K1016, K570 |
| E1BN43 | Ankyrin repeat domain 28 | ANKRD28 | S1011 | K525 |
| E1BP31 | Ankyrin repeat domain 42 | ANKRD42 | S509 | K509 |
| E1B717 | Armadillo repeat containing 10 | ARMC10 | S43, S45 | K205, K111, K122 |
| F1N4A1 | Armadillo repeat containing 12 | ARMC12 | S91, S48 | K136 |
| P33097 | Aspartate aminotransferase, cytoplasmic | GOT1 | S312 | K290 |
| A6H758 | C11H9ORF9 protein | SPACA9 | S107 | K99 |
| Q32L61 | Calcium binding tyrosine phosphorylation regulated | CABYR | S331, S335, S240, S243, S118, S427, S367 | K5, K242 |
| Q28068 | Calicin | CCIN | S391, S12, S58, S97 | K400 |
| E1BM27 | Calmin | CLMN | S930 | K545, K157, K58, K176 |
| P00517 | cAMP‐dependent protein kinase catalytic subunit alpha | PRKACA | S140, T198 | K30, K48, K280, K310 |
| P00514 | cAMP‐dependent protein kinase type I‐alpha regulatory subunit | PRKAR1A | S377, S374 | K221, K366, K260, K133 |
| Q32KS3 | Capping actin protein of muscle Z‐line alpha subunit 3 | CAPZA3 | S274, Y289 | K89 |
| A6QQM1 | CCNY protein | CCNY | S19, S272 | K247 |
| Q3ZBU2 | CDGSH iron–sulfur domain‐containing protein 1 | CISD1 | S41 | K77, K53, K66 |
| F1MKR7 | Cilia and flagella associated protein 77 | CFAP77 | T178 | K51 |
| F1N343 | Coiled‐coil domain containing 136 | CCDC136 | S651, S330, S574, S322, S541, S411, S299, S645 | K627, K436, K369, K424, K347, K438, K137, K881, K416, K378, K473 |
| F1MEL4 | Coilin | COIL | S259 | K477, K460 |
| G3N176 | Desumoylatingisopeptidase 1 | DESI1 | S25 | K18 |
| Q0III6 | DnaJ homolog subfamily B member 6 | DNAJB6 | S15 | K60 |
| E1BLB4 | Dynein axonemal heavy chain 17 | DNAH17 | T174 | K1543 |
| E1B9R5 | Dynein axonemal heavy chain 8 | DNAH8 | S136, S1199, S89, S138, S81 | K4081, K578, K2401, K2205, K4551, K1675 |
| F1N7B9 | Endophilin‐B1 | SH3GLB1 | S200 | K29 |
| G3X6E2 | Family with sequence similarity 166 member A | FAM166A | S147, S187 | K63, K61 |
| F1MB15 | Fibrous sheath interacting protein 2 | FSIP2 | S4686, S5359, S1340, S3081, S6426, S6169, S6476, S4716, S6723, S6076, S5976, S3099, S6576, S6245, S4414, S4657, S4176, S6095, S1466, S5213, S4379, S3186, S4546, S6226, S4497, S6726, S4382, S908, S4124, S6715, S5322 | K4039, K4098, K3257, K3854, K3673, K6502, K4323, K4058, K4671, K683, K4382 |
| A6QLL8 | Fructose‐bisphosphate aldolase | ALDOA | S36, S354 | K348, K200, K111 |
| Q3ZBY4 | Fructose‐bisphosphate aldolase | ALDOC | S45 | K111 |
| Q8MJN0 | FUN14 domain‐containing protein 2 | FUNDC2 | S44, S54, S33, S152 | K63, K162, K151, K160, K62, K171 |
| F1MUX6 | Glutathione S‐transferase | GSTM3 | S143, S77 | K170, K161, K181, K71, K40, K199, K193, K38, K132, K19, K31, K55 |
| Q0VCS8 | Glutathione S‐transferase, theta 3 | GSTT3 | S25 | K190, K23 |
| P10096 | Glyceraldehyde‐3‐phosphate dehydrogenase | GAPDH | S149 | K261, K213 |
| Q2KJE5 | Glyceraldehyde‐3‐phosphate dehydrogenase, testis‐specific | GAPDHS | S269, S300, S210, T296 | K274, K221, K204, K278, K318, K167 |
| Q76LV2 | Heat shock protein HSP 90‐alpha | HSP90AA1 | S52 | K58, K69, K74, K84, K185, K420, K408, K112, K209, K191, K224, K284, K293, K616 |
| E1BNY9 | HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase | HUWE1 | S3761, S3758, S1852 | K1733 |
| F1MWF0 | Huntingtin interacting protein 1 | HIP1 | S764 | K621, K660, K775, K977, K604, K527, K575, K927, K730, K1033 |
| Q32KR7 | Hypothetical LOC539526 | SAXO1 | S330, S450, T67 | K135 |
| A7MBC5 | IARS protein | IARS | S827 | K1000, K1042 |
| F1MCM7 | IQ motif containing N | IQCN | S711, S737, S764, S724, S1096 | K888 |
| E1BM42 | KIAA1468 | RELCH | S49, S52, S176 | K873 |
| E1BNS9 | l‐Lactate dehydrogenase | LDHC | S321, S161, S105 | K232 |
| F1MLS4 | Membrane spanning 4‐domains A14 | MS4A14 | S349, S283 | K492, K547, K541 |
| F1N1D6 | Metaxin‐1 | MTX1 | S50 | K41 |
| F1MZU2 | N‐ethylmaleimide sensitive factor, vesicle fusing ATPase | NSF | S437 | K728, K161, K529, K266, K293, K469 |
| E1BCV4 | Nucleoporin 98 | NUP98 | S607 | K796, K756, K807 |
| E1BHV9 | Ornithine decarboxylase antizyme 3 | OAZ3 | S55, S9, Y11 | K17 |
| Q2T9U2 | Outer dense fiber protein 2 | ODF2 | S37, S74, S139, S237, S101, S129, S261, S244, S645, S58, S632, S73, T172, T177, T31, T39 | K259, K239, K253, K259, K609 |
| E1BJM5 | PAS domain containing serine/threonine kinase | PASK | S158 | K1035, K1046, K218, K1131 |
| F1N0B2 | Phospholipid‐transporting ATPase | LOC536660 | S855 | K152, K704, K229 |
| A4FUZ3 | Proteasome (prosome, macropain) 26S subunit, ATPase, 1 | PSMC1 | S244 | K178 |
| F1MKX4 | Proteasome activator complex subunit 4 | PSME4 | S293, S294 | K918, K636, K56, K36, K1501, K574 |
| Q3ZCK9 | Proteasome subunit alpha type‐4 | PSMA4 | S7 | K54 |
| Q58DN4 | Protein phosphatase methylesterase 1 | PPME1 | S42 | K328 |
| E1BC58 | RAB2B, member RAS oncogene family | RAB2B | S67, S202 | K120, K29 |
| F1N058 | Ropporin‐1 | ROPN1 | S106, S56, S62 | K6, K73, K139, K23, K199 |
| F1MX05 | Saccharopine dehydrogenase‐like oxidoreductase | SCCPDH | S406 | K220, K257 |
| Q0VD22 | Serine/threonine‐protein kinase 33 | STK33 | S87 | K373 |
| Q3SZY8 | Small membrane A‐kinase anchor protein | ‐‐‐ | S24, S40 | K20 |
| Q32L54 | Sperm‐associated antigen 6 | SPAG6 | S490 | K457, K438 |
| F1MI43 | Sperm surface protein Sp17 | SPA17 | S54 | K52 |
| F1MUB8 | Spermatogenesis and centriole‐associated 1 | SPATC1 | S252, S330, S337, S238, S312 | K421, K38, K483, K471, K430 |
| Q3SZQ3 | Spermatogenesis‐associated protein 19, mitochondrial | SPATA19 | S133, S84, S142, S91, S37, S59, S26, S116, S138, S107, S82 | K83 |
| Q3T0K2 | T‐complex protein 1 subunit gamma | CCT3 | S414 | K128, K507 |
| Q3ZCI9 | T‐complex protein 1 subunit theta | CCT8 | S261, S317 | K326, K62 |
| A0JNM2 | Thioredoxin | TXNDC8 | S51 | K72 |
| E1BJD3 | Transmembrane protein 190 | TMEM190 | S127 | K131 |
| E1BCX4 | Tripartite motif containing 42 | TRIM42 | S124 | K697, K680 |
| P81947 | Tubulin alpha‐1B chain | ‐‐‐ | S48 | K370 |
| Q3MHM5 | Tubulin beta‐4B chain | TUBB4B | S115, S40 | K350, K122, K58, K336, K362, K324, K154, K216, K297 |
| F1ME38 | Ubiquitin like modifier activating enzyme 6 | UBA6 | S697 | K503, K729, K537, K978, K796, K628, K644, K739, K709, K368, K1003, K746, K800, K58, K531, K714, K652, K687, K871, K544 |
| A3KMV5 | Ubiquitin‐like modifier‐activating enzyme 1 | UBA1 | S810 | K635 |
| F1MHK9 | Uncharacterized protein | LOC524391 | S823 | K1627, K692, K761 |
| F1ML59 | Uncharacterized protein | NT5C1B | S75, S53, S129, S68, S131, S43, S202, T151, T195 | K225 |
| F1N3R5 | Uncharacterized protein | AQP7 | S14 | K24, K15 |
| F1N6K8 | Uncharacterized protein | LOC789612 | S17 | K253 |
| F6Q0K7 | Uncharacterized protein | SPATA32 | S337 | K206, K258 |
| G3X752 | Vesicle‐associated membrane protein 3 | VAMP3 | S61 | K38, K69, K45 |
| P68002 | Voltage‐dependent anion‐selective channel protein 2 | VDAC2 | S115 | K120, K285 |