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Cycloaddition of 2 a with 3 a catalyzed by evolved mbpNB‐1′ variants. Reaction conditions: 2 a (0.125 mM), 3 a (2.0 mM) and AgNO3 (1.0 mM) in a maltose elution buffer (25 mM maltose, 100 mM AcOH, 50 mM MES, 2 % 1,4‐dioxane, pH 5.5) containing mbpNB‐1′ variants (ca. 2 μM), 37 °C for 72 h.
