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. 2020 Oct 29;45(2):368–381. doi: 10.1002/cbin.11493

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© 2020 The Authors. Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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graphic file with name CBIN-45-368-g002.jpg — Downregulation of MARCH1 can not inhibit proliferation and apoptosis in CRC cell lines. (a) Western blot analysis assay showed that the expression level of MARCH1 decreased after using two siRNAs for 48 h compared with siNC in CRC cell lines. (b) Microscope images of the CRC cells of MARCH1 siRNA interference for 48 h using ×100 image lens (scale bar: 200 µm). (c) The cell viability of the DLD‐1 and sw480 cells transfected with MARCH1 siRNAs and siNC at 48 h using CCK‐8 assay. (d) The cell growth curve of the DLD‐1 and sw480 cells transfected with MARCH1 siRNAs and siNC at 0, 24, 48, and 72 h using CCK‐8 assay.(e) The cell apoptosis rate of the DLD‐1 and sw480 cells transfected with MARCH1 siRNAs and siNC at 48 h. Results were presented as mean ± SD of three independent experiments. CCK‐8, cell counting kit‐8; CRC, colorectal cancer; MARCH1, membrane‐associated RING‐CH‐1; SD, standard deviation; siRNA, small interfering RNA

graphic file with name CBIN-45-368-g003.jpg — Downregulation of MARCH1 can not inhibit proliferation and apoptosis in CRC cell lines. (a) Western blot analysis assay showed that the expression level of MARCH1 decreased after using two siRNAs for 48 h compared with siNC in CRC cell lines. (b) Microscope images of the CRC cells of MARCH1 siRNA interference for 48 h using ×100 image lens (scale bar: 200 µm). (c) The cell viability of the DLD‐1 and sw480 cells transfected with MARCH1 siRNAs and siNC at 48 h using CCK‐8 assay. (d) The cell growth curve of the DLD‐1 and sw480 cells transfected with MARCH1 siRNAs and siNC at 0, 24, 48, and 72 h using CCK‐8 assay.(e) The cell apoptosis rate of the DLD‐1 and sw480 cells transfected with MARCH1 siRNAs and siNC at 48 h. Results were presented as mean ± SD of three independent experiments. CCK‐8, cell counting kit‐8; CRC, colorectal cancer; MARCH1, membrane‐associated RING‐CH‐1; SD, standard deviation; siRNA, small interfering RNA