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. 2020 Oct 29;45(2):368–381. doi: 10.1002/cbin.11493

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© 2020 The Authors. Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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graphic file with name CBIN-45-368-g008.jpg — Effect of 5‐FU on CRC cell migration, invasion, EMT, and PI3K/AKT pathway. (a) Wound healing assay demonstrated that the migration ability in CRC cells after 24 and 48 h of 5‐FU treatment (0, 10, and 20 µM) using ×40 image lens (scale bar: 200 µm). (b) Transwell assay demonstrated that the invasion ability in CRC cells after 48 h of 5‐FU treatment (0, 10, and 20 µM) (scale bar: 200 µm). (c) Western blot analysis assay showed the protein expression of MARCH1, EMT, PI3K/AKT pathway molecules in CRC cells after 48 h of 5‐FU treatment (0, 10, and 20 µM). Results were presented as mean ± SD of three independent experiments. *p < .05, **p < .01, ***p < .001. 5‐FU, 5‐fluorouracil; CRC, colorectal cancer; EMT, epithelial–mesenchymal transition; SD, standard deviation

graphic file with name CBIN-45-368-g009.jpg — Effect of 5‐FU on CRC cell migration, invasion, EMT, and PI3K/AKT pathway. (a) Wound healing assay demonstrated that the migration ability in CRC cells after 24 and 48 h of 5‐FU treatment (0, 10, and 20 µM) using ×40 image lens (scale bar: 200 µm). (b) Transwell assay demonstrated that the invasion ability in CRC cells after 48 h of 5‐FU treatment (0, 10, and 20 µM) (scale bar: 200 µm). (c) Western blot analysis assay showed the protein expression of MARCH1, EMT, PI3K/AKT pathway molecules in CRC cells after 48 h of 5‐FU treatment (0, 10, and 20 µM). Results were presented as mean ± SD of three independent experiments. *p < .05, **p < .01, ***p < .001. 5‐FU, 5‐fluorouracil; CRC, colorectal cancer; EMT, epithelial–mesenchymal transition; SD, standard deviation