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. 2021 Feb 16;30:0963689721992066. doi: 10.1177/0963689721992066

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Macaque iPSCs form a teratoma in autologous transplantation but not in MHC-matched allogeneic transplantation. Monkeys #733 and #1497 were transplanted with CMF1/1 -1 cells in subcutaneous tissue of the shoulders and were observed for 12 weeks. (A) Schema of the transplantation. Autologous iPSCs, CMF1/1 -1 cells at passage 24, and iMSCs at passage 5 derived from CMF1/1 -1 cells were injected into subcutaneous tissue of the right shoulder (ˆ) and left shoulder (•) of macaque #733 with 50% Matrigel, respectively. (B) Schema of the transplantation. MHC-matched allogeneic iPSCs, CMF1/1 -1 cells at passage 41, and iMSCs at passage 4 derived from CMF1/1 -1 cells were respectively injected into subcutaneous tissue of the right shoulder (ˆ) and left shoulder (•) of macaque #1497 with 50% Matrigel. (C) The right shoulder of the iPSC transplantation site was swollen at 6 weeks after transplantation (circled), while the left shoulder of the iMSC transplantation site (arrows) was not swollen. (D) Ultrasonography revealed a heteroechoic mass (circled) in subcutaneous tissue on the scapula of the right shoulder but not that of the left shoulder 3 months after transplantation. (E) Neither shoulder of the cell transplantation site (arrows) was swollen 3 months after transplantation. (F) Ultrasonography revealed no mass in subcutaneous tissue in both shoulders 3 months after transplantation. (G) Gross appearance of the teratoma in the right shoulder of monkey #733. (H) HE-stained section of the teratoma in monkey #733. The magnification was ×40. (I) HE-stained section of biopsy specimens of the left shoulder of monkey #733 at 3 months after transplantation. The magnification was ×40. (J, K, and L) Immunohistochemistry of the teratoma. The magnifications of AFP (endoderm), αSMA (mesoderm), and β3-tubulin (ectoderm) were ×200, ×200 and ×400, respectively. HE: hematoxylin and eosin; iPSC: induced pluripotent stem cell; MHC: major histocompatibility complex.