Figure 3.

Immunological reaction of the transplanted cells in monkeys #733 and #1497. (A, B) iPSCs were cultured without MEFs to avoid the absorption of MEF antigens for a flow cytometric assay. CMF1/1 -1 cells, iMSCs, and MEFs were incubated with the plasma of monkeys #733 (A) and #1497 (B) as a primary antibody. Fluorescein-conjugated anti-monkey IgG was used to detect IgG specific for the cells in plasma as a secondary antibody. Filled histograms indicate cells stained with the plasma collected before transplantation. Colored lines indicate cells stained with the plasma collected after transplantation. *: Passage number. C, D, E, and F: ELISPOT assay of IFN-γ (C, E) and IL-2 (D, F). Peripheral blood cells in autologous transplantation in monkey #733 (C, D) and in MHC-matched allogeneic transplantation in monkey #1497 (E, F) were cultured with the lysate of indicated cells. The experiments were usually performed in duplicate wells for each condition. Results are shown as S.I. HE: hematoxylin and eosin; IFN: interferon; IgG: immunoglobulin G; IL: interleukin; iPSC: induced pluripotent stem cell; MEF: mouse embryonic fibroblast; MHC: major histocompatibility complex; S.I.: stimulation indices.