Figure 7.

Knockdown of SND1 or YWHAB reversed the effects of SMARCA5 siRNA on the proliferation, invasion, and apoptosis of cervical cancer cells. The HeLa cells were transfected with SMARCA5 siRNA alone or together with SND1 siRNA or YWHAB siRNA. (A-B) The expression of SMARCA5 was detected by RT-qPCR. The expression of SND1 and YWHAB was detected by western blotting, and the quantification was performed using Image J software. n = 3. *P < 0.05, **P < 0.01, one-way ANOVA. (C-E) The changes in cell proliferation, invasion, and apoptosis were detected by Cell Counting Kit-8, Transwell, and Annexin V-FITC/PI detection kit, respectively. n = 3. **P < 0.01, one-way ANOVA. (F) The expression of apoptosis-related proteins was detected by western blotting, and the quantification was performed using Image J software. n = 3. *P < 0.05, one-way ANOVA.

ANOVA: analysis of variance; FITC: fluorescein isothiocyanate; NC: negative control; OD: optical density; PI: propidium iodide.