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. 2021 Jan;24(1):116–122. doi: 10.22038/ijbms.2020.49486.11313

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The serum anti-tetanus toxoid (TT) immunoglobulin G titers of immunized rabbits with different vaccine formulations by nasal administration. Animals were inoculated at days 0, 14, and 28 with one of the following formulations: TT solution; trimethyl chitosan (TMC) containing TT+cross-linked dextran microspheres (CDM); and TMC (TT)+Lactose (Lac). The intramuscular injection of Alum TT was administrated as a positive control. At days 42, sera were collected to identify the IgG titers by end-point titration using the ELISA technique. Results present the mean±SD (n=4)