Figure 4.

The nasal lavage anti-tetanus toxoid (TT) immunoglobulin A titers of vaccinated rabbits followed by nasal delivery of formulations. Rabbits were immunized at days 0, 14, and 28 with one of the following formulations: TT solution; trimethyl chitosan (TMC) containing TT + cross-linked dextran microspheres (CDM);+ cross-linked dextran microspheres (CDM); and TMC (TT) + Lactose (Lac). At days 42, the nasal lavages were collected to determine the sIgA titers by end-point titration using the ELISA method. Data were expressed as the mean±SD (n=4)

*Significant difference between TT solution and test groups *(P<0.05), **(P<0.01), and ***(P<0.001)

+Significant difference between test groups

+(P<0.05), ++(P<0.01), and +++(P<0.001)