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Transfection rate of HEK293 and PBMCs. (A) HEK293 cell transfection with CRISPR vectors. (B) PBMCs before transfection (left), efficiency of PBMCs transfection was about 50%, after 72 hr (middle), efficiency of PBMCs transfection was about 80%, after 120 hr (right)
HEK293: human embryonic kidney cells; PBMCs: peripheral blood mononuclear cells; CRISPR: clustered regularly interspaced short palindromic repeats
