Table 4. Differently expressed genes identified by RT2-profilers arrays in the intestinal mucosa of 35 versus 14 days old Normal Birth Weight (NBW) piglets.
| Arrays/Genesa | Description | Foldb | p-valuesc | FDRd |
|---|---|---|---|---|
| Oxidative stress | ||||
| AOX1 | Aldehyde oxidase-like | -2.17 | 0.017 | 0.120 |
| CCL5 | Chemokine (C-C motif) ligand 5 | 2.10 | <0.01 | 0.049 |
| FTH1 | Ferritin, heavy polypeptide 1 | -2.48 | <0.01 | 0.031 |
| GPX2 | Glutathione peroxidase 2 (gastrointestinal) | 4.82 | <0.01 | 0.065 |
| GPX4 | Glutathione peroxidase 4 (phospholipid hydroperoxidase) | -2.19 | <0.01 | 0.058 |
| HMOX1 | Heme oxygenase (decycling) 1 | -5.31 | 0.036 | 0.106 |
| SFTPD | Surfactant protein D | 2.57 | 0.023 | 0.130 |
| SIRT2 | Sirtuin 2 | -2.19 | <0.01 | 0.058 |
| UCP2 | Uncoupling protein 2 (mitochondrial, proton carrier) | -2.62 | 0.046 | 0.186 |
| Mito. energy | ||||
| ATP4B | ATPase, H+/K+ exchanging, beta polypeptide | 9.00 | <0.01 | <0.01 |
| EDN1 | Endothelin 1 | -2.21 | 0.025 | 0.323 |
| NDUFB4 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4-like | -2.27 | <0.01 | 0.168 |
| Cytokines | ||||
| BMP3 | Bone morphogenetic protein 3 | -2.58 | <0.01 | 0.024 |
| C5 | Complement component 5 | 2.42 | <0.01 | <0.01 |
| CCL19 | Chemokine (C-C motif) ligand 19 | 2.38 | 0.015 | 0.026 |
| CCL21 | Chemokine (C-C motif) ligand 21 | -2.98 | 0.028 | 0.078 |
| CCL28 | Chemokine (C-C motif) ligand 28 | 3.37 | 0.027 | 0.078 |
| CXCL10 | Chemokine (C-X-C motif) ligand 10 | 6.36 | <0.01 | <0.01 |
| CXCL9 | Chemokine (C-X-C motif) ligand 9 | 8.18 | <0.01 | 0.026 |
| IFN-ALPHA-4 | Interferon-alpha-4 | -2.69 | <0.01 | <0.01 |
| IFN-ALPHA-5 | Interferon, alpha 5 | -2.06 | <0.01 | 0.013 |
| IL12B | Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) | 10.41 | <0.01 | 0.018 |
| IL17A | Interleukin 17A | 4.68 | <0.01 | <0.01 |
| IL17F | Interleukin 17F | 5.66 | 0.028 | 0.078 |
| IL18 | Interleukin 18 (interferon-gamma-inducing factor) | 2.10 | <0.01 | 0.019 |
| IL2 | Interleukin 2 | 18.86 | 0.021 | 0.067 |
| IL21 | Interleukin 21 | 264.39 | <0.01 | <0.01 |
| IL22 | Interleukin 22 | 10.10 | 0.022 | 0.067 |
| INHBA | Inhibin, beta A | -2.98 | <0.01 | <0.01 |
| CNTF | Ciliary neurotrophic factor-like | -2.27 | <0.01 | 0.026 |
| TNFSF13B | Tumor necrosis factor (ligand) superfamily, member 13b | 29.53 | <0.01 | <0.01 |
| Apoptosis | ||||
| APAF1 | Apoptotic peptidase activating factor 1 | -2.58 | 0.044 | 0.104 |
| BCL2A1 | Apoptosis-associated speck-like protein containing a CARD-like | 2.94 | <0.01 | 0.015 |
| LOC100154044 | BCL2-like 2 | -2.83 | 0.029 | 0.093 |
| CASP1 | Caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) | 2.09 | <0.01 | 0.014 |
| CASP10 | Caspase 10, apoptosis-related cysteine peptidase | -2.06 | 0.012 | 0.046 |
| CIDEB | Cell-death-inducing DNA-fragmentation-factor-like effector B | -2.63 | <0.01 | 0.014 |
| IGF1R | Insulin-like growth factor 1 receptor | -4.88 | <0.01 | 0.022 |
| LOC100522011 | Apoptosis-associated speck-like protein containing a CARD-like | 2.09 | <0.01 | 0.012 |
| LTA | Lymphotoxin alpha (TNF superfamily, member 1) | -4.11 | 0.042 | 0.101 |
| MTL5 | Metallothionein-like 5, testis-specific (tesmin) | 2.24 | 0.032 | 0.093 |
aRT2-profilers used were oxidative stress, mitochondrial energy metabolism (mito. energy), cytokines and chemokines (cytokines) and apoptosis. Each array included 84 genes.
bFold change (2-ΔΔCt) is the normalized gene expression (2-ΔCt)) at d 23 divided the normalized gene expression (2-ΔCt) at d 14. Fold-change values greater than one indicates a positive- or an up-regulation and values less than one indicate a negative or down-regulation.
cThe p values are calculated based on a Student’s t-test of the replicate (2-ΔCt) values for each gene from NBW piglets of both ages.
dThe FDR is the False Discovery Rate adjusted p-values.