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. 2021 Feb 19;16(2):e0247396. doi: 10.1371/journal.pone.0247396

Table 3. Membrane-binding potential and cellular localization of CPPs.

Epitope Protein-binding Potential (Boman index) Hydrophobic moment (μH) Membrane-binding potential (D factor) Cellular localization by TMHMM Server Total probability of N-in by TMHMM Server
S protein
NLTTRTQLPP 2.49 0.148 0.469 Inside 0.47745
RFQTLLALHR 2.3 0.342 1.147 Inside 0.48730
YLQPRTFLLK 1.09 0.225 0.8724 Outside 0.34806
SVYAWNRKRI 3.24 0.224 1.196 Inside 0.89106
YAWNRKRISN 4.31 0.162 1.142 Inside 0.91633
AWNRKRISNC 4.17 0.259 1.234 Inside 0.92814
WNRKRISNCV 3.95 0.300 1.273 Inside 0.91952
RQIAPGQTGK 2.55 0.081 0.736 Inside 0.71347
YNYLYRLFRK 2.96 0.690 1.641 Inside 0.74845
YLYRLFRKSN 3.28 0.567 1.525 Inside 0.69159
YRLFRKSNLK 3.82 0.275 1.5796 Inside 0.75816
RLFRKSNLKP 3.81 0.322 1.623 Inside 0.61242
RKSNLKPFER 4.98 0.368 1.337 Inside 0.70344
KKSTNLVKNK 3.24 0.355 1.655 Inside 0.87353
KSTNLVKNKC 2.56 0.377 1.345 Inside 0.85159
HADQLTPTWR 2.99 0.468 0.606 Inside 0.56152
YQTQTNSPRR 5.62 0.323 0.964 Inside 0.89727
TQTNSPRRAR 6.36 0.219 1.196 Inside 0.92515
TNSPRRARSV 5.49 0.292 1.265 Inside 0.85479
NSPRRARSVA 5.05 0.329 1.300 Inside 0.82719
PRRARSVASQ 4.94 0.302 1.275 Inside 0.83300
KQIYKTPPIK 1.5 0.194 1.173 Inside 0.77001
SQILPDPSKP 1.67 0.404 0.381 Outside 0.15396
RLITGRLQSL 2.07 0.502 0.897 Inside 0.47579
M protein
NRNRFLYIIK 3.1 0.346 1.316 Inside 0.81684
RNRFLYIIKL 1.95 0.326 1.297 Inside 0.66588
YIIKLIFLWL -2.91 0.488 0.790 Outside 0.20334
KLIFLWLLWP -2.66 0.304 0.616 Outside 0.07090
FIASFRLFAR 1.08 0.522 1.152 Outside 0.32626
ASFRLFARTR 3.62 0.343 1.313 Inside 0.67733
SFRLFARTRS 4.14 0.366 1.335 Inside 0.64273
FRLFARTRSM 3.56 0.350 1.320 Inside 0.67490
RLFARTRSMW 3.63 0.589 1.546 Inside 0.78030
FARTRSMWSF 2.67 0.277 0.921 Inside 0.62650
HGTILTRPLL 0.41 0.254 0.569 Outside 0.24888
GAVILRGHLR 1.2 0.323 0.964 Outside 0.42609
RIAGHHLGRC 2.43 0.423 1.059 Inside 0.64698
YSRYRIGNYK 3.99 0.141 1.123 Inside 0.89352
N protein
PQNQRNAPRI 4.74 0.243 0.889 Inside 0.80935
ERSGARSKQR 6.67 0.143 1.124 Inside 0.89938
RSGARSKQRR 7.48 0.114 1.757 Inside 0.94959
SGARSKQRRP 5.99 0.188 1.497 Inside 0.85655
GARSKQRRPQ 6.2 0.185 1.494 Inside 0.89829
ARSKQRRPQG 6.2 0.185 1.494 Inside 0.89829
RSKQRRPQGL 5.89 0.280 1.584 Inside 0.83224
SKQRRPQGLP 4.4 0.245 1.221 Inside 0.60745
KQRRPQGLPN 4.72 0.292 1.265 Inside 0.69009
RRPQGLPNNT 4.53 0.432 1.067 Inside 0.62344
QIGYYRRATR 4.54 0.383 1.351 Inside 0.93350
IGYYRRATRR 5.48 0.445 1.740 Inside 0.95251
GYYRRATRRI 5.48 0.564 1.852 Inside 0.95251
YYRRATRRIR 7.07 0.593 2.209 Inside 0.98065
YRRATRRIRG 6.96 0.565 2.183 Inside 0.96774
RRATRRIRGG 6.85 0.478 2.101 Inside 0.94656
RATRRIRGGD 6.23 0.365 1.334 Inside 0.90958
TRRIRGGDGK 5.38 0.314 1.286 Inside 0.85699
RIRGGDGKMK 3.95 0.321 1.293 Inside 0.80713
GKMKDLSPRW 2.76 0.523 1.153 Inside 0.53414
SQASSRSSSR 5.39 0.171 0.821 Inside 0.70911
ASSRSSSRSR 6.33 0.114 1.097 Inside 0.79316
SSRSSSRSRN 7.18 0.180 1.159 Inside 0.81855
SRSSSRSRNS 7.18 0.183 1.162 Inside 0.81855
RSSSRSRNSS 7.18 0.179 1.158 Inside 0.81855
SSSRSRNSSR 7.18 0.165 1.145 Inside 0.81855
SSRSRNSSRN 7.5 0.192 1.171 Inside 0.86592
RSRNSSRNST 7.42 0.194 1.173 Inside 0.90573
GSSRGTSPAR 3.89 0.156 0.807 Inside 0.52191
AALALLLLDR -0.63 0.125 0.118 Outside 0.15140
ALALLLLDRL -0.95 0.288 0.271 Outside 0.09169
KKSAAEASKK 3.03 0.277 1.251 Inside 0.85101
KSAAEASKKP 2.48 0.385 1.023 Inside 0.66436
SAAEASKKPR 3.42 0.284 0.928 Inside 0.71820
AAEASKKPRQ 3.63 0.306 0.948 Inside 0.79675
AEASKKPRQK 4.36 0.248 1.224 Inside 0.86032
EASKKPRQKR 6.04 0.165 1.475 Inside 0.92101
ASKKPRQKRT 5.61 0.096 1.740 Inside 0.94440
SKKPRQKRTA 5.61 0.060 1.706 Inside 0.94440
KKPRQKRTAT 5.53 0.087 1.732 Inside 0.96166
KPRQKRTATK 5.53 0.094 1.738 Inside 0.96166
PRQKRTATKA 4.79 0.202 1.510 Inside 0.94133
RQKRTATKAY 4.81 0.311 1.423 Inside 0.96930
RQGTDYKHWP 3.88 0.330 0.641 Inside 0.65834
FPPTEPKKDK 3.17 0.088 0.413 Outside 0.35377
PPTEPKKDKK 4.02 0.225 0.872 Inside 0.58321
PTEPKKDKKK 4.58 0.236 1.212 Inside 0.80442
TEPKKDKKKK 5.13 0.110 1.527 Inside 0.92302
EPKKDKKKKA 4.7 0.147 1.458 Inside 0.90887
PKKDKKKKAD 4.89 0.184 1.493 Inside 0.90457
KKDKKKKADE 5.57 0.133 1.115 Inside 0.93664
TQALPQRQKK 3.84 0.308 0.950 Inside 0.86179
ALPQRQKKQQ 4.14 0.243 1.219 Inside 0.86346
ORF3a
SASKIITLKK 0.94 0.243 1.219 Inside 0.75288
ASKIITLKKR 2.09 0.314 1.452 Inside 0.86807
SKIITLKKRW 2.04 0.492 1.784 Inside 0.86095
KIITLKKRWQ 2.25 0.485 1.777 Inside 0.90139
IITLKKRWQL 1.21 0.320 1.292 Inside 0.76749
KKRWQLALSK 2.65 0.335 1.636 Inside 0.79484
KRWQLALSKG 2 0.407 1.704 Inside 0.62646
VRIIMRLWLC 0.01 0.429 1.064 Inside 0.63104
RIIMRLWLCW 0.18 0.600 1.226 Inside 0.64902
IIMRLWLCWK -0.74 0.517 1.148 Inside 0.61977
IMRLWLCWKC -0.38 0.347 0.987 Inside 0.68839
MRLWLCWKCR 1.59 0.156 1.137 Inside 0.81046
RLWLCWKCRS 2.17 0.178 1.158 Inside 0.77912
LWLCWKCRSK 1.23 0.223 1.200 Inside 0.74360
WLCWKCRSKN 2.39 0.080 1.065 Inside 0.85315
LCWKCRSKNP 2.62 0.198 1.176 Inside 0.78747
CWKCRSKNPL 2.62 0.236 1.212 Inside 0.78747
KCRSKNPLLY 2.5 0.134 1.116 Inside 0.67216
Orf1ab
IKRSDARTAP 4.15 0.217 0.864 Inside 0.82802
KRSDARTAPH 5.11 0.039 0.696 Inside 0.81819
PVAYRKVLLR 1.58 0.313 1.285 Inside 0.60465
VAYRKVLLRK 2.13 0.208 1.516 Inside 0.80830
AYRKVLLRKN 3.2 0.224 1.531 Inside 0.85177
YRKVLLRKNG 3.29 0.241 1.547 Inside 0.79673
RKVLLRKNGN 3.94 0.200 1.508 Inside 0.80930
KVLLRKNGNK 3 0.234 1.540 Inside 0.77882
FEIKLAKKFD 1.45 0.412 0.718 Inside 0.48733
KTIQPRVEKK 3.75 0.289 1.262 Inside 0.88737
TIQPRVEKKK 3.75 0.161 1.141 Inside 0.88737
IQPRVEKKKL 3 0.103 1.087 Inside 0.78283
SGLKTILRKG 1.53 0.531 1.491 Inside 0.54410
LKTILRKGGR 2.68 0.625 1.91 Inside 0.71606
KTILRKGGRT 3.43 0.472 1.765 Inside 0.84956
GNFKVTKGKA 1.51 0.141 1.123 Inside 0.68622
FKVTKGKAKK 2.05 0.306 1.938 Inside 0.87336
KVTKGKAKKG 2.25 0.248 1.884 Inside 0.88617
KGKAKKGAWN 2.1 0.165 1.475 Inside 0.82016
GGAKLKALNL -0.25 0.094 0.748 Outside 0.30336
SKGLYRKCVK 2.39 0.571 1.859 Inside 0.83410
KGLYRKCVKS 2.39 0.574 1.861 Inside 0.83410
GLYRKCVKSR 3.32 0.456 1.750 Inside 0.85872
GLLMPLKAPK -0.87 0.305 0.947 Outside 0.15005
QRKQDDKKIK 6.07 0.223 1.2 Inside 0.95960
RKQDDKKIKA 5.33 0.227 1.204 Inside 0.94962
KQDDKKIKAC 3.71 0.420 1.056 Inside 0.92486
DITFLKKDAP 1.64 0.215 0.202 Inside 0.41432
MLAKALRKVP 0.61 0.641 1.595 Inside 0.57064
LAKALRKVPT 1.1 0.661 1.613 Inside 0.61542
EAKTVLKKCK 1.95 0.508 1.469 Inside 0.87393
AKTVLKKCKS 1.61 0.525 1.815 Inside 0.87056
KTVLKKCKSA 1.61 0.517 1.808 Inside 0.87056
KSAFYILPSI -0.7 0.344 0.654 Outside 0.26827
KAIVSTIQRK 2.18 0.455 1.419 Inside 0.89354
STIQRKYKGI 2.68 0.547 1.506 Inside 0.87849
TIQRKYKGIK 2.9 0.533 1.823 Inside 0.93039
IQRKYKGIKI 2.15 0.356 1.656 Inside 0.90786
GARFYFYTSK 1.8 0.190 0.839 Inside 0.58982
ARYMRSLKVP 2.58 0.412 1.378 Inside 0.72252
GIEFLKRGDK 2.68 0.472 0.775 Inside 0.47568
DNLKTLLSLR 2.21 0.363 0.672 Outside 0.37423
YMSALNHTKK 1.94 0.373 1.012 Inside 0.74444
SALNHTKKWK 2.48 0.407 1.374 Inside 0.76830
ALNHTKKWKY 2.15 0.311 1.283 Inside 0.81149
LNHTKKWKYP 2.34 0.291 1.264 Inside 0.69869
NHTKKWKYPQ 3.38 0.362 1.331 Inside 0.82885
HTKKWKYPQV 2.31 0.217 1.194 Inside 0.78945
KKPASRELKV 3.1 0.317 1.289 Inside 0.71408
KPASRELKVT 2.8 0.356 0.996 Inside 0.66033
YTPSFKKGAK 1.7 0.425 1.391 Inside 0.60188
PSFKKGAKLL 0.44 0.488 1.450 Outside 0.28608
FKKGAKLLHK 1.12 0.639 1.923 Inside 0.56438
KKGAKLLHKP 1.42 0.544 1.833 Inside 0.55042
KGAKLLHKPI 0.37 0.590 1.546 Inside 0.43639
WCIRCLWSTK 0.93 0.223 0.870 Inside 0.79516
CIRCLWSTKP 1.17 0.298 0.941 Inside 0.70751
ANYAKPFLNK 1.64 0.408 1.045 Inside 0.53508
TNIVTRCLNR 3.3 0.562 1.190 Inside 0.89302
CTFTRSTNSR 4.67 0.140 0.792 Inside 0.87592
TCMMCYKRNR 3.74 0.228 1.205 Inside 0.96377
MCYKRNRATR 5.42 0.232 1.539 Inside 0.97415
CYKRNRATRV 5.25 0.084 1.399 Inside 0.97070
YKRNRATRVE 6.06 0.094 1.078 Inside 0.95678
KRNRATRVEC 5.92 0.077 1.062 Inside 0.96153
RNRATRVECT 5.62 0.032 0.690 Inside 0.95230
RDLSLQFKRP 4.02 0.454 1.088 Inside 0.49113
SLQFKRPINP 2.32 0.319 0.961 Outside 0.39508
HNIALIWNVK 0.05 0.188 0.507 Inside 0.60778
LSEQLRKQIR 4.19 0.686 1.307 Inside 0.75607
QLRKQIRSAA 3.64 0.527 1.487 Inside 0.86742
LRKQIRSAAK 3.64 0.621 1.906 Inside 0.89068
RKQIRSAAKK 4.68 0.451 2.075 Inside 0.95767
KQIRSAAKKN 3.86 0.413 1.709 Inside 0.93797
QIRSAAKKNN 3.97 0.319 1.291 Inside 0.92032
AAKKNNLPFK 1.84 0.198 1.176 Inside 0.63148
KKNNLPFKLT 1.96 0.238 1.214 Inside 0.54962
NNWLKQLIKV 0.87 0.728 1.347 Inside 0.65372
LAYYFMRFRR 3 0.469 1.432 Inside 0.71897
AYYFMRFRRA 3.31 0.303 1.276 Inside 0.82144
YYFMRFRRAF 3.19 0.478 1.441 Inside 0.72131
FMRFRRAFGE 3.75 0.600 1.226 Inside 0.52960
MRFRRAFGEY 4.06 0.504 1.135 Inside 0.70022
KEMYLKLRSD 3.29 0.265 0.580 Inside 0.65269
YNRYLALYNK 2.25 0.500 1.132 Inside 0.74756
RYLALYNKYK 2.14 0.392 1.360 Inside 0.80198
FRKMAFPSGK 1.83 0.313 1.285 Inside 0.47019
TANPKTPKYK 2.67 0.009 0.998 Inside 0.77069
ANPKTPKYKF 2.12 0.169 1.149 Inside 0.63687
PKTPKYKFVR 2.72 0.292 1.595 Inside 0.73005
KTPKYKFVRI 2.23 0.323 1.624 Inside 0.82704
RWFLNRFTTT 3.09 0.360 0.999 Inside 0.70898
FQSAVKRTIK 2.37 0.618 1.573 Inside 0.83635
SEVVLKKLKK 1.44 0.508 1.469 Inside 0.64932
VVLKKLKKSL 0.27 0.612 1.897 Inside 0.55852
VLKKLKKSLN 1.34 0.757 2.034 Inside 0.62971
KKLKKSLNVA 1.65 0.534 1.824 Inside 0.74490
DAAMQRKLEK 3.62 0.368 0.677 Inside 0.81844
AAMQRKLEKM 2.51 0.518 1.148 Inside 0.83287
MQRKLEKMAD 3.56 0.646 0.939 Inside 0.82292
YKQARSEDKR 6.37 0.233 0.879 Inside 0.92834
KQARSEDKRA 6.17 0.232 0.879 Inside 0.92248
QARSEDKRAK 6.17 0.186 0.835 Inside 0.92248
MLFTMLRKLD 0.93 0.562 0.860 Outside 0.39049
QDLKWARFPK 2.82 0.337 0.978 Inside 0.59730
DLKWARFPKS 2.61 0.356 0.996 Inside 0.48806
LKWARFPKSD 2.61 0.331 0.972 Inside 0.48806
KGFCDLKGKY 1.44 0.467 1.1 Inside 0.55047
GVSAARLTPC 0.6 0.109 0.432 Inside 0.44779
GFAKFLKTNC 0.53 0.530 1.160 Inside 0.51312
KTNCCRFQEK 4.2 0.333 0.974 Inside 0.92234
PHISRQRLTK 4.17 0.167 1.147 Inside 0.78638
HISRQRLTKY 4.18 0.190 1.169 Inside 0.87931
ISRQRLTKYT 3.97 0.208 1.186 Inside 0.90259
SRQRLTKYTM 4.23 0.231 1.208 Inside 0.90877
RQRLTKYTMA 3.71 0.210 1.188 Inside 0.92213
GERVRQALLK 3.11 0.401 1.038 Inside 0.66110
RVRQALLKTV 2.37 0.490 1.452 Inside 0.78888
KPYIKWDLLK 0.84 0.143 0.794 Inside 0.49602
RLKLFDRYFK 3.4 0.622 1.577 Inside 0.61889
KLFDRYFKYW 2.18 0.889 1.499 Inside 0.59390
FPFNKWGKAR 2.16 0.338 1.309 Inside 0.52642
KWGKARLYYD 2.5 0.177 0.827 Inside 0.72287
YAISAKNRAR 3.52 0.076 1.061 Inside 0.91112
AISAKNRART 3.76 0.037 1.024 Inside 0.91919
KNRARTVAGV 3.19 0.315 1.287 Inside 0.88250
NRQFHQKLLK 3.55 0.436 1.401 Inside 0.73407
RQFHQKLLKS 3.23 0.495 1.457 Inside 0.65716
RIMASLVLAR 0.84 0.180 0.829 Inside 0.57528
RNLQHRLYEC 4.25 0.519 0.819 Inside 0.77699
RLYECLYRNR 4.73 0.430 1.065 Inside 0.85428
SLRCGACIRR 3.3 0.356 1.326 Inside 0.83575
RCGACIRRPF 3.15 0.633 1.587 Inside 0.77458
CGACIRRPFL 1.17 0.422 1.058 Inside 0.52389
GACIRRPFLC 1.17 0.269 0.913 Inside 0.52389
ACIRRPFLCC 1.13 0.416 1.052 Inside 0.66539
CIRRPFLCCK 1.87 0.493 1.455 Inside 0.75457
IRRPFLCCKC 1.87 0.298 1.271 Inside 0.75457
RRPFLCCKCC 2.23 0.347 1.317 Inside 0.81002
MSYYCKSHKP 1.92 0.271 0.915 Inside 0.68047
ANTCTERLKL 2.61 0.348 0.988 Inside 0.76670
SWEVGKPRPP 2.33 0.123 0.446 Outside 0.22968
VGKPRPPLNR 3.21 0.403 1.370 Inside 0.41222
GKPRPPLNRN 4.28 0.261 1.236 Inside 0.48502
KALKYLPIDK 0.89 0.154 0.805 Inside 0.53339
DKCSRIIPAR 3.45 0.418 1.054 Inside 0.82505
KCSRIIPARA 2.4 0.376 1.344 Inside 0.83189
CSRIIPARAR 3.34 0.425 1.391 Inside 0.84740
SRIIPARARV 3.06 0.289 1.262 Inside 0.80072
RIIPARARVE 3.4 0.233 0.879 Inside 0.79867
SVVNARLRAK 2.88 0.144 1.125 Inside 0.81132
VVNARLRAKH 3.00 0.138 1.120 Inside 0.83250
VNARLRAKHY 3.42 0.100 1.084 Inside 0.86059
NARLRAKHYV 3.42 0.057 1.043 Inside 0.86059
PAPRTLLTKG 1.3 0.242 0.888 Outside 0.35023
APRTLLTKGT 1.55 0.313 0.955 Inside 0.54170
FNSVCRLMKT 1.75 0.603 1.229 Inside 0.71012
FLGTCRRCPA 1.92 0.606 1.232 Inside 0.60210
DNKLKAHKDK 4.42 0.269 0.913 Inside 0.80851
KLKAHKDKSA 3.04 0.217 1.194 Inside 0.79044
FLTRNPAWRK 3.25 0.256 1.231 Inside 0.70721
RNPAWRKAVF 2.9 0.167 1.147 Inside 0.75095
GIPKDMTYRR 3.86 0.181 0.830 Inside 0.81853
DMTYRRLISM 3.01 0.334 0.645 Inside 0.78592
GNPKAIKCVP 0.47 0.301 0.944 Inside 0.53539
WNTFTRLQSL 2.04 0.432 0.737 Inside 0.51104
ELWAKRNIKP 2.54 0.248 0.894 Inside 0.69594
LWAKRNIKPV 1.46 0.307 1.279 Inside 0.70434
WAKRNIKPVP 1.95 0.435 1.4 Inside 0.69474
RNIKPVPEVK 2.64 0.365 1.004 Inside 0.65866
LLIGLAKRFK 0.06 0.556 1.514 Outside 0.34306
GLAKRFKESP 2.55 0.567 1.195 Inside 0.41824
KMQRMLLEKC 2.25 0.534 1.164 Inside 0.77714
VLRQWLPTGT 0.84 0.442 0.747 Inside 0.37448
DMSKFPLKLR 2.29 0.438 1.073 Outside 0.39910
MSKFPLKLRG 1.33 0.425 1.391 Outside 0.33087
SKFPLKLRGT 1.82 0.312 1.284 Outside 0.37440
KFPLKLRGTA 1.3 0.337 1.308 Outside 0.41667
MILSLLSKGR 0.43 0.383 1.021 Outside 0.30296
LLSKGRLIIR 1.32 0.419 1.385 Outside 0.44682
GRLIIRENNR 4.91 0.336 0.977 Inside 0.81939
RLIIRENNRV 4.6 0.443 1.078 Inside 0.85576
ORF6
LIIKNLSKSL 0 0.601 1.227 Outside 0.38147
ORF7a
HVYQLRARSV 2.87 0.205 0.853 Inside 0.75260
QLRARSVSPK 3.69 0.081 1.066 Inside 0.70446
RARSVSPKLF 2.84 0.344 1.314 Inside 0.52495
RSVSPKLFIR 2.53 0.158 1.139 Inside 0.51018
ITLCFTLKRK 1.21 0.272 1.246 Inside 0.72907
TLCFTLKRKT 1.96 0.280 1.254 Inside 0.78296
LCFTLKRKTE 2.38 0.341 0.981 Inside 0.71141
ORF8
SKWYIRVGAR 2.48 0.303 1.276 Inside 0.82389
KWYIRVGARK 2.7 0.208 1.516 Inside 0.89799
YIRVGARKSA 2.54 0.273 1.247 Inside 0.83725