Table 1.

Comparision of clonal methods

	Single cell transplantation	Lentiviral barcoding	Endogenous SB barcoding
Requirement of ex vivo manipulation	Yes	Yes	No
Nº of cells that can be simultaneously tracked in one mouse	1	In practice, under 100,000 (due to library production constraints and sequencing/cost limitations).	In practice, under 1M (due to sequencing/cost limitations).
Cost (per standard experiment)	Low	Intermediate	High
Efficiency	Variable	Variable	Low (up to 50%)
Target population for tracing	Only HSCs	Any cell	Any cell
Requirement of cell sorting for analysis of results	No.	Depends (if barcodes are expressed or not)	Yes
False positives	Extremely rare	Rare (depends on library diversity, target population, and possible contaminations)	Rare (mostly due to contaminations and whole genome amplification)
False negatives	Extremely rare.	Rare	Very frequent (due to poorly performing Tn capture techniques)
Possible use for investigating human biology	Yes	Yes	No