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. Author manuscript; available in PMC: 2021 Feb 19.
Published in final edited form as: Anal Chem. 2021 Feb 2;93(6):3172–3180. doi: 10.1021/acs.analchem.0c04592

Figure 2.

Figure 2.

Dye-displacement assay for colorimetric detection of THC. (A) Spectra of ETC at various concentrations of THC (0, 0.25, 0.5, 1, 1.5, 2, 2.5, 3.5, 5, 10, and 20 μM) and (B) corresponding calibration curves. Inset shows response at low concentrations of target. (C) Cross-reactivity of 5 μM THC-COOH, CBN and THCA; 25 μM CBD, CBDA, CBG, CBGA, UR-144, and XLR-11; and other small-molecule drugs at a concentration of 100 μM. Cross-reactivity was calculated relative to the signal gain produced by 5 μM THC. Inset shows photographs of the assay for selected specificity tests. Error bars represent the standard deviation of measurements from three individual experiments. Abbreviation: α-PVP = α-pyrrolidinopentiophenone, Meth = methamphetamine.