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. Author manuscript; available in PMC: 2021 Feb 19.
Published in final edited form as: Immunohorizons. 2020 Dec 18;4(12):825–836. doi: 10.4049/immunohorizons.2000073

Figure 1. Early prophylactic inhibition of RIP2 reduces lung pathology in an acute house dust mite (HDM) model of asthma.

Figure 1.

A.) WT C57BL/6 mice were subjected to an acute HDM asthma model and were administered either regular chow or chow containing RIP2 inhibitor (GSK583 at 30mg/kg/day) for the 5 days during and surrounding the initial exposure to HDM (red arrows). Mice were euthanized on day 14 and lungs were harvested, fixed in 10% formalin, and embedded in paraffin. B.) Consecutive sections were stained with either H&E, PAS, or Trichrome. Severity of lung pathology was scored based on C.) inflammatory index, D.) mucus production, E.) lumen narrowing, F.) epithelial thickness, and G.) collagen deposition and was performed by a blinded observer. Data are presented as scatterplots with bars where bar heights represent means ± SEM. Filled in circles represent individual datapoints for vehicle/control chow-treated mice and open circles represent individual datapoints for GSK583-treated mice. Data are pooled from 3 independent experiments for a total of n=9 mice per group. Statistical analysis was performed using an unpaired, two-tailed Student’s t-test. *** = p<0.001, **** = p<0.0001.