Figure 5. Mice undergoing a chronic HDM asthma model exhibit improved lung pathology with early RIP2 inhibition but minimal effects on HDM-specific antibody responses.

A.) WT C57BL/6 mice were subjected to a chronic HDM asthma model and were administered either regular chow or chow containing RIP2 inhibitor (GSK583 at 30mg/kg/day) for the 5 days during and surrounding the initial exposure to HDM (red arrows). On day 28, mice were euthanized and lungs were harvested, fixed in 10% formalin, and embedded in paraffin. B.) Consecutive sections were stained with either H&E, PAS, or Trichrome. Severity of lung pathology was blindly scored based on C.) inflammatory index, D.) remodeling index and E.) mucus production. F.) Levels of HDM-specific total IgG and IgG₁ antibodies in the serum of vehicle chow or RIP2 inhibitor (GSK583) chow-treated mice subjected to a chronic HDM asthma model as measured by ELISA. Data are presented as scatterplots with bars where bar heights represent means ± SEM. Filled in circles represent individual datapoints for vehicle/control chow-treated mice and open circles represent individual datapoints for GSK583-treated mice. Data are pooled from 3 independent experiments for a total of n=9 mice per group. Statistical analysis was performed using an unpaired, two-tailed Student’s t-test. ***= p<0.001, ****= p<0.0001