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. 2021 Feb 8;17(2):e1008992. doi: 10.1371/journal.ppat.1008992

Fig 1. CVB3 VP1 affects cell proliferation and blocks cell cycle at the G1/S phase.

Fig 1

(A) The numbers of adherent HPAC cells in different groups were counted using Image Pro Plus (IPP) software. (B) HPAC cells were transfected by pBud-VP1 and pBud plasmids, and the cell proliferation was evaluated using the CCK-8 assay. (C) The activity of DNA replication was examined using EdU incorporation in HPAC cells. (D) G1/S phase arrest induced by VP1 transfection. HPAC cells were treated with 36-hour plasmid transfection (pBud and pBud-VP1) within the treatment of double-thymidine, and collected at 0, 6 and 15 hpi separately, then cells were analyzed by flow cytometry. Values are shown as the mean ± SEM of three independent experiments. (*P<0.05, **P<0.01).