Fig 4. ΔtamA undergoes enhanced clearance from the gut following administration of CBBP or fecal microbiota transplantation (FMT).

(A, B) Mice were treated with either (A) vancomycin or (B) ampicillin in drinking water and inoculated with 1:1 mixture of wild type and ΔtamA strains. The density of each strain in feces was determined over 14 days. Mean ± SEM of log₁₀CI (competitive index) is shown. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001, by one-sample t test on log10 transformation of CI. (C–E) Ampicillin-treated mice were mono-colonized with wild type (open circles) or ΔtamA (closed circles) strains and clearance by PBS, CBBPSCSK [36,37], or CBBPCon was compared. The arrows indicate when PBS or either bacterial consortia were administered. Bar graphs represent geometric means and the dotted line indicates limit of detection. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001, by unpaired multiple t test on log10 transformation. (F, G) V+M (Vancomycin and metronidazole)–treated mice were mono-colonized with wild type or ΔtamA strains and clearance by PBS or FMT prepared from naïve mice was compared. The arrows indicate when PBS or FMT were administered, and bar graphs represent geometric means. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001, by unpaired multiple t test on log10 transformation.