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. 2021 Feb 19;7(8):eabe0174. doi: 10.1126/sciadv.abe0174

Fig. 4. NMR TRF1 supports improved TRF1 and TIN2 nuclear localization and limits telomere elongation.

Fig. 4

(A to D) Representative images (A) and quantification (B and D) of colocalization between flag-TRF1 foci (green) and myc-TIN2 (purple) (B); quantification of total number of flag-TRF1 foci in nucleus without and with ectopic myc-TIN2 expression (C) and of total number of myc-TIN2 foci in nucleus (D) in MEFs with SV40-LT TRF1F/F following KO of endogenous TRF1 by CRE and rescue by ectopic NMR or mouse TRF1 orthologs under atmospheric oxygen or at 3% oxygen. (E) Telomere length distribution averages are represented by the shape of each beanplot, with small vertical lines that represent the mean of telomere length for each metaphase spread. Statistics: Student and ANOVA tests, n ≥ 30 samples, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001. AU, arbitrary units.