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. 2021 Feb 19;7(8):eabf0717. doi: 10.1126/sciadv.abf0717

Fig. 5. The mitochondrial methylome is highly conserved from fly to mouse and human.

Fig. 5

(A) Schematic workflow to confirm the untargeted mitochondrial fly methylome in three species by targeted PRM-based LC-MS/MS. Heavy isotope-labeled synthesized peptide standards were spiked into endogenous peptide samples. (B) Confidence scoring of selected methyl groups targeted with synthesized standards in Dm. (C) Conservation status of 203 methylated Dm residues in human protein homologs. nc, nonconserved amino acid. major ticks are 10, and minor ticks are 2 residues. (D) Scores of conserved residues in human (Hs) and mouse (Mm) fibroblasts as in (C). Listing in PhosphoSitePlus (68) as black squares in the literature column. The best score of at least three experiments is shown in (B) and (D). unmod, unmodified; mod, modified.