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. 2021 Feb 19;7(8):eabf0717. doi: 10.1126/sciadv.abf0717

Fig. 6. ISC assembly is sensitive to mitoSAM loss.

Fig. 6

(A) Interaction score of p223l transcriptomics and proteomics. Methylated proteins are shown in dark red. Gene names to methylated targets with an absolute interaction score of >1 in at least one dataset. (B) Mitochondrial aconitase activity (n = 4). (C) Transmission electron micrographs. Arrows indicate suspected iron deposits in null and p223l mitochondria. Scale bar, 0.5 μm. (D) Total cellular iron levels in 4-day-old Dm larvae (n = 4). (E) Interaction site of R72 in HsNFS1 with ISD11 and 4-PPE in gray [Protein Data Bank (PDB): 5USR]. (F) Cellular iron levels in fly models overexpressing DmNfs1 constructs (n = 3). Control is da-GAL4/+. (G) Immunoenrichment of DmNfs1.R77F-FLAG over DmNfs1.R77K-FLAG (n = 4). Black dots, mitochondrial; orange dot, DmNfs1. Significant proteins with known relation to iron metabolism or OXPHOS are labeled. (H) BN-PAGE immunoblot with an antibody against NFS1 in cells overexpressing HsNFS1-FLAG constructs (representative image, n = 3). The missing band in HsNFS1.R72K is marked. Graphs show means + SD. *P < 0.05, **P < 0.01, and ***P < 0.001 with Dunnett’s test against wDah (B and C) or control (F) or false discovery adjusted P value (G). (A) to (D), (F), and (G) performed on 4-day-old larvae.