Figure 5: The NTE is critical for inhibition.

(a) Degradation of ^SFGFP-ssrA (5 μM) by ClpAP (0.2 μM ClpA₆, 0.4 μM ClpP₁₄) alone (solid black circles) or in the presence of 1 μM ClpS (empty black circles), 1 μM H₆-DHFR-ClpS (empty red circles), or 1 μM H₆-DHFR-ClpS and 10 μM MTX (solid red circles). Data are representative of 3 independent experiments. The dotted line is a visual cue to indicate the biphasic nature of the degradation kinetics in presence of H₆-DHFR-ClpS. (b) Inhibition of degradation of ^SFGFP-ssrA (5 μM) by ClpAP (1 μM ClpA₆, 2 μM ClpP₁₄) with ClpS alone (3 μM or 6 μM), ClpS^ΔN17 alone (6 μM), or a mixture of ClpS (3 μM) and ClpS^ΔN17 (3 μM). Curves are representative of 3 independent experiments.