Figure 7: Effect of NTE length on V_max for degradation.

V_max (blue circles) for ClpAP (0.4 μM ClpA₆, 0.8 μM ClpP₁₄) degradation of different concentrations of ^SFGFP-ssrA were determined by Michaelis-Menten analysis of experiments performed in the presence of NTE truncation variants of ClpS (2.4 μM). Values are average ± 1 SD (n = 3). ATPase rates (grey squares) for ClpAP in presence of ClpS NTE truncation variants from Roman-Hernandez et al [21]. Values were normalized to the wt. ClpA and plotted allowing direct comparison of the changes in V_max and ATPase rates resulting from varying the ClpS NTE length. The dotted lines represent the values obtained in the presence of wt. ClpS.