Figure 4. The Hippo pathway regulates Kibra (Kib) abundance via a putative degron motif.

(A–F’) Mer somatic mosaic clones in wing discs expressing either UASp-Kib-GFP (A-C') or UASp-Kib^S677A-GFP (D–F’) with the nub>Gal4 driver. Note that wild-type Kib-GFP is significantly elevated in Mer clones both apically and basally, while Kib^S677A-GFP is only slightly stabilized apically and is not affected basally. Yellow arrows in C and F point to presumed Kib aggregates due to increased abundance. All scale bars=20 μm. (G–H) Quantification of clone/control ratio of apical (G) and basal (H) Kib-GFP fluorescence. All quantification is represented as the mean ± SEM; asterisks above the plots show p-values between the transgenes; asterisks inside each bar show p-values for each transgene with respect to 1; n=number of clones (no more than two clones per wing disc were used for quantification). Statistical comparison was performed using Mann–Whitney U-test. (I–N’) hpo somatic mosaic clones in wing discs expressing either UASp-Kib-GFP (I–K’) or UASp-Kib^S677A-GFP (L–N’) with the nub>Gal4 driver. Note that wild-type Kib-GFP levels are significantly elevated in hpo clones both apically and basally, while Kib^S677A-GFP is stabilized apically but depleted basally in hpo clones. (O–P) Quantification of clone/control ratio of apical (O) and basal (P) Kib-GFP fluorescence.