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. 2021 Feb 8;10:e62326. doi: 10.7554/eLife.62326

Figure 7. Kibra (Kib) degradation is patterned by mechanical tension in the wing pouch to control proportional growth.

(A–B’) Grayscale images of the wing pouch, which produces the adult wing blade, expressing UASp-Kib-GFP (A) or UASp-KibS677A-GFP (B) at identical genomic locations under the nub>Gal4 driver. Corresponding heatmap intensity images are shown in (A’) and (B’). Note that KibS677A-GFP displays a more uniform distribution across the pouch than wild-type Kib-GFP. (C) Quantification of aspect ratios of adult wings expressing nub>Gal4 alone or with UASp-Kib-GFP and UASp-KibS677A-GFP. The color-coded segments in the wing image represent the wing length (orange), distal width (green), and proximal width (red). (D) Quantification of the length of proximal (S1) or distal (S2) wing region with respect to total wing length in wings expressing nub>Gal4 alone or with UASp-Kib-GFP and UASp-KibS677A-GFP; p-cv=posterior crossvein. All quantification is represented as the mean ± SEM; n=number of wings (one wing per fly). Statistical comparison was performed using the one-way ANOVA test followed by Tukey’s HSD test. (E–F’) Kib-GFP levels are elevated in rapidly proliferating UAS-bantam clones. (G–G’’’) Increased Kib abundance is more pronounced at the center of the wing pouch (yellow arrowhead) than at its periphery (white arrow). (H–I’) KibΔWW1-GFP levels do not change in bantam-expressing clones. All scale bars=20 μm.

Figure 7.

Figure 7—figure supplement 1. Kibra (Kib) degradation is not uniform across the pouch region of the wing imaginal disc.

Figure 7—figure supplement 1.

(A) A grayscale and heatmap image of a wing imaginal disc expressing endogenous Kib::GFP. (B–B’) Additional examples of wing discs expressing Kib-GFP or KibS677A-GFP under UASp control using the nub>Gal4 driver focused on the wing pouch, which produces the adult wing blade. (C) To generate the intensity profiles in (C), rectangular selections of equal length were drawn on each disc either in the anterior or posterior region (same side was used for each pair). Intensity values from UASp-Kib-GFP discs were normalized to the maximum value of UASp-KibS677A-GFP discs. (D) Intensity profiles of nine pairs of wing imaginal discs expressing UASp-Kib-GFP or UASp-KibS677A-GFP under the nub>Gal4 driver. In each case, wild-type Kib intensity drops more severely from the distal (middle) to proximal (most dorsal or ventral) part of the wing pouch.