Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | kibra | DOI: 10.1016/j.devcel.2009.12.012
DOI: 10.1016/j.devcel.2009.12.011 DOI: 10.1016/j.devcel.2009.12.013 |
FLYB: FBgn0262127 | |
| Genetic reagent (D. melanogaster) | Kib::GFP | DOI: 10.1016/j.devcel.2017.02.004 | ||
| Genetic reagent (D. melanogaster) | Mer4 19AFRT | LaJeunesse et al., 1998 | ||
| Genetic reagent (D. melanogaster) | exe1 40AFRT | PMID: 8269855 | ||
| Genetic reagent (D. melanogaster) | 19AFRT sd47M | Wu et al., 2008 | ||
| Genetic reagent (D. melanogaster) | hpoBF33 42DFRT | Jia et al., 2003 | ||
| Genetic reagent (D. melanogaster) | ban3-GFP | DOI: 10.1242/dev.070367 | ||
| Genetic reagent (D. melanogaster) | UAS-Mer RNAi | DOI: 10.1016/j.devcel.2017.02.004 | ||
| Genetic reagent (D. melanogaster) | UAS-sav RNAi | Bloomington Drosophila Stock Center | BL 28006 | |
| Genetic reagent (D. melanogaster) | UAS-hpo RNAi | Vienna Drosophila Resource Center | VDRC 104169 | |
| Genetic reagent (D. melanogaster) | UAS-wts RNAi | Vienna Drosophila Resource Center | VDRC 106174 | |
| Genetic reagent (D. melanogaster) | UAS-ex RNAi | Vienna Drosophila Resource Center | VDRC 109281 | |
| Genetic reagent (D. melanogaster) | UAS-crb RNAi | Vienna Drosophila Resource Center | VDRC 39177 | |
| Genetic reagent (D. melanogaster) | UAS-yki RNAi (III) | Vienna Drosophila Resource Center | VDRC 40497 | |
| Genetic reagent (D. melanogaster) | UAS-slimb RNAi | Bloomington Drosophila Stock Center | BL 33898 | |
| Genetic reagent (D. melanogaster) | UAS-Cul1 RNAi | Bloomington Drosophila Stock Center | BL 29520 | |
| Genetic reagent (D. melanogaster) | UAS-SkpA RNAi | Bloomington Drosophila Stock Center | BL 32870 | |
| Genetic reagent (D. melanogaster) | UAS-mahj RNAi | Bloomington Drosophila Stock Center | BL 34912 | |
| Genetic reagent (D. melanogaster) | UAS-Nedd4 RNAi | Bloomington Drosophila Stock Center | BL 34741 | |
| Genetic reagent (D. melanogaster) | UAS-POSH RNAi | Bloomington Drosophila Stock Center | BL 64569 | |
| Genetic reagent (D. melanogaster) | UAS-POSH | Bloomington Drosophila Stock Center | BL 58990 | |
| Genetic reagent (D. melanogaster) | UAS-Su(dx) RNAi | Bloomington Drosophila Stock Center | BL 67012 | |
| Genetic reagent (D. melanogaster) | UAS-Herc4 | DOI: 10.1371/journal.pone.0131113 | ||
| Genetic reagent (D. melanogaster) | UAS-Smurf RNAi | Bloomington Drosophila Stock Center | BL 40905 | |
| Genetic reagent (D. melanogaster) | UAS-Fbxl7 RNAi | Vienna Drosophila Resource Center | VDRC 108628 | |
| Genetic reagent (D. melanogaster) | UAS-ft RNAi | Bloomington Drosophila Stock Center | BL 34970 | |
| Genetic reagent (D. melanogaster) | UAS-ds RNAi | Vienna Drosophila Resource Center | VDRC 36219 | |
| Genetic reagent (D. melanogaster) | UAS-dachs-V5 | DOI: 10.1242/dev.02427 | ||
| Genetic reagent (D. melanogaster) | UAS-Tao1 RNAi | Vienna Drosophila Resource Center | VDRC 17432 | Previously used in DOI: 10.1016/j.devcel.2011.08.028 |
| Genetic reagent (D. melanogaster) | UAS-mats RNAi | Bloomington Drosophila Stock Center | BL 34959 | |
| Genetic reagent (D. melanogaster) | UAS-Pez RNAi | Bloomington Drosophila Stock Center | BL 33918 | |
| Genetic reagent (D. melanogaster) | Ey>Flp 19AFRT Ubi-GFP; Ubi-RFP 42DFRT | DOI: 10.1016/j.devcel.2013.04.021 | ||
| Genetic reagent (D. melanogaster) | Ft-GFP | VDRC 318477 | ||
| Genetic reagent (D. melanogaster) | Ds:GFP | Brittle et al., 2012 | ||
| Genetic reagent (D. melanogaster) | Ubi-Kib-GFP-FLAG 86Fb | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | UASp-Kib-GFP-FLAG 86Fb | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | UASp-KibS677A-GFP-FLAG 86Fb (this study) | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-Kib-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-KibΔWW1-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-KibΔWW2-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-KibΔWW1 and 2-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-Kib1-857-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-Kib484-1288-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-Kib858-1288-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-KibΔCC1-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Genetic reagent (D. melanogaster) | Ubi-KibΔCC2-GFP-FLAG VK37 | This paper | See Materials and methods section | |
| Antibody | anti-Ex (Guinea pig polyclonal) | DOI: 10.1016/j.cub.2006.02.063 | RRID:AB_2568722 | Tissue staining (1:5000) |
| Antibody | anti-FLAG (Mouse monoclonal) | Sigma Aldrich | Cat#F1804; RRID:AB_262044 | IB (1:20,000) |
| Antibody | anti-Sd (Guinea pig polyclonal) | Guss et al., 2013 | RRID:AB2567874 | Tissue staining (1:1000) |
| Antibody | anti-GFP (Guinea pig polyclonal) | DOI: 10.1091/mbc.E19-07-0387 | NA | IP (1:1250) |
| Antibody | anti-GFP (Rabbit polyclonal) | Michael Glotzer (University of Chicago) | NA | IB (1:5000) |
| Antibody | anti-Hpo (mouse polyclonal) | DOI: 10.1016/j.devcel.2017.02.004 | NA | IB (1:5000) |
| Antibody | anti-HA (Rabbit polyclonal) | Santa Cruz | Cat#sc-805; RRID:AB_631618 | IB (1:5000) |
| Antibody | anti-Myc 9B11 (Mouse monoclonal) | Cell Signaling | Product #2276 | IP (1:1000) IB (1:40,000) |
| Antibody | anti-V5 (Mouse monoclonal) | GenScript | Cat# A01724-100 | IB (1:2500) |
| Antibody | anti-alpha tubulin (Mouse monoclonal) | Sigma Aldrich | Cat# T 9026 | IB (1:2500) |
| Cell line (D. melanogaster) | S2-DGRC | Cherbas Lab, Indiana University | RRID:CVCL_TZ72 | https://dgrc.bio.indiana.edu/product/View?product=6 |