Fig. 2.

Portions of 800-MHz ¹H NMR spectra of mouse heart tissue extracts obtained without degassing, immediately after sample preparation (Figs. 2a and 2c) and 24 h after preparation (Figs. 2b and 2d). Note, that while the acetyl-CoA level is unaltered, the CoA, NADH, and NADPH levels are reduced drastically with a concomitant increase of the CoA-S-S-glutathione, NAD⁺, and NADP⁺ levels, respectively, 24 h after sample preparation. Portions of spectra of mouse heart tissue extracts with degassing of both the NMR tubes and D₂O buffer using helium gas obtained immediately after sample preparation (Figs. 2e and 2g) and 24 h after preparation (Figs. 2f and 2h). Note that the levels of the CoA, acetyl-CoA, and CoA-S-S-glutathione, NADH, NADPH, NAD⁺, and NADP⁺ are all unaltered even 24 h after sample preparation, which indicates no oxidation of CoA, NADH, and NADPH