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. 2021 Feb 8;58(4):5. doi: 10.3892/ijo.2021.5185

Figure 4.

Figure 4

ST3GAL6-AS1 interacts with hnRNPA2B1 by stem loop 5. (A) ChIRP-MS revealed that hnRNPA2B1 protein binds to ST3GAL6-AS1. (B) RNA pull-down assay confirmed that hnRNPA2B1 protein bind to ST3GAL6-AS1. (C) RIP method was used to verify the interaction of hnRNPA2B1 and ST3GAL6-AS1 reversely. (D) Secondary structure prediction of ST3GAL6-AS1. (E) RNA pull-down assay showed that hnRNPA2B1 protein specifically bind to ST3GAL6-AS1 stem loop 5. *P<0.05 vs. IgG. hnRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; RIP, RNA immunoprecipitation.