Figure 5.

ST3GAL6-AS1 recruits hnRNPA2B1 in nucleus to promote ST3GAL6 translation in cytoplasm. (A) hnRNPA2B1 protein translocated to cell cytoplasm when ST3GAL6-AS1 knockdown in MM cell lines. (B) Efficiency of hnRNPA2B1-knockdown measured by reverse transcription quantitative PCR and western blotting. (C) Knockdown of hnRNPA2B1 significantly decreased the degradation of ST3GAL6 mRNA when MM cells were treated with acti- nomycin D. 18S rRNA was the normalizer. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. respective control. hnRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; MM, multiple myeloma; si, small interfering; NC, negative control.