Figure 2.
Intravital microscopy conducted on male and female C57BL/6 mice fed a chow (male n = 6, female n = 5) or high-fat (male n = 4, female n = 5) diet for 14–16 weeks. Intravital microscopy was performed in mice who were in a fed state prior to the procedure (cohort 1). Vessel diameter of 2A arteriole after 45-minute equilibrium period (baseline) and at maximal dilation with addition of SNP 10−2 in male (A) and female (B) mice. Acetylcholine (C, D) and phenylephrine (E, F) dose response curves illustrating percent diameter change from baseline arteriole diameter in male (C, E) and female (D, F) mice. ∗p < 0.05, ∗∗P < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.