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. 2021 Feb 2;24(2):102125. doi: 10.1016/j.isci.2021.102125

Figure 2.

Figure 2

OT promotes the proliferation of AML12 cell line and enhances the growth of mouse hepatic organoid

(A) Growth curve of AML12 cell line under different concentrations of OT treatment (10−8 Mol/L～10−4Mol/L) respectively for 0h, 4h, 8h, 12hr, 16hr, 20hr, 24hr.

(B)3-D mouse hepatic organoids from 2- to 3-month-old C57BL/6 mice were cultured in matrigel and administered with PBS (Control) or OT (10−8 Mol/L～10−4Mol/L) for 0 hr, 12 hr, 36 hr, 72 hr (5-6 wells/group). The pictures showed the size of mouse hepatic organoids (×40). At least 4 image fields per each sample were quantified. Bottom: The quantification of the liver organoids size in the 2- to 3-month-old C57BL/6 mice by Image-Pro Plus 6.0. Original magnifications: ×40 (B); scale bar: 200μm. Values represent the mean ± Standard error of mean (SEM). ∗∗p < 0.01; ∗∗∗p < 0.001; t test. OT: oxytocin; OD: optical density (450 nm).