Table 2.
SKP2 mutants display a range of binding affinities to cyclin A. Kd values were determined by isothermal titration calorimetry.
| Skp2 | Residue changes | Cyclin A Kd ± (nM)1 |
|---|---|---|
| Wild-type | None | 298 ± 17 |
| SKP2N_4A | L32A L33A S39A L41A | 5290 ± 215 |
| SKP2N_6A | W22A W24A L32A L33A S39A L41A | ND2 |
| SKP2N_Δ20 | N-terminal 20 residues deleted | 507 ± 295 |
| SKP2N_Δ30 | N-terminal 30 residues deleted | ND |
1
Carried out at 25 °C with a starting concentration of 10 μM CDK2-cyclin A in the cell and 120 μM SKP1-SKP2N in the syringe.
2
Could not be determined. Experiments were carried out using a Malvern Microcal PEAQ-ITC. Kd is the derived average of two independent biological replicates and SD of the population.