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. 2021 Feb 19;12:1151. doi: 10.1038/s41467-021-21407-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Projection of cells combined from clusters identified as microglia (MG), monocytes/macrophages (Mo/MΦ), and BAMs from all groups. b Top ten differentially expressed genes for the three main identified cell populations, new marker candidates are in bold. c, d Feature plots depicting genes highly expressed in MG (c) and MoMΦ (d). e Flow cytometric analysis of the distribution of Tmem119 and Gal-3 protein markers within CD11b⁺ cells and projection of Tmem119⁺ and Gal-3⁺ cells onto CD45/CD11b graphs, dot plots demonstrate percentages of Tmem119⁺ and Gal-3⁺ cells within CD45^hi and CD45^lo groups (n = 8, 4 males, and 4 females, two-sided Mann–Whitney U test, mean ± SD, *** < 0.001, Tmem119 Pv = 0.0002, Gal-3 Pv = 0.0002). f Feature plots depicting distribution of the expression of genes discriminating monocytes (Mo), monocyte–macrophage intermediate (intMoMΦ), and macrophage (MΦ) subpopulations. g Density plots demonstrating the expression level of markers discriminating the Mo/MΦ subpopulations. h Flow cytometry analysis of CD49d and PD-L1 proteins within CD11b⁺ cells and their projection onto CD11b/CD45 graphs, dot plots demonstrate percentages of CD49d⁺ and PD-L1⁺ cells within CD45^hi and CD45^lo groups (n = 4, 2 males, and 2 females; two-sided Mann–Whitney U test, mean ± SD, * < 0.05, CD49d Pv = 0.0286, PD-L1 Pv = 0.0286). i Flow cytometry analysis of the distribution of the markers discriminating Mo/MΦ subpopulations within CD11b⁺CD45^hi cells, dot plots demonstrate percentage of CD11b⁺CD45^hi cells that belong to the defined populations (n = 4, 2 males, and 2 females; two-sided Mann–Whitney U test, mean ± SD, * < 0.05, Ly6C CD49d Pv = 0.0286, Ly6C PD-L1 Pv = 0.0286). j UMAP plot showing clusters of Mo/MΦ subpopulations.