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. 2021 Feb 19;12:1151. doi: 10.1038/s41467-021-21407-w

Fig. 3. Tumor-derived microglia and macrophages form separate cell populations.

Fig. 3

a UMAP plots demonstrate the distribution of CD11b+ cells from naive and tumor-bearing mice. b Distribution of MG and Mo/MΦ “signature” gene scores (presented as an average of expression of the selected genes). c Density plots of MG and Mo/MΦ scores across MG and Mo/MΦ populations demonstrating no overlap of a specific “signature” between the two cell populations, two-sided Wilcoxon signed-rank test. d Cell hierarchical clustering according to the expression of reported macrophage markers demonstrating bimodal cell distribution, two-sided Fisher’s exact test. e Immunohistochemical staining for microglia (Tmem119+ and Gal-3−) and Mo/MΦ (Tmem119− and Gal-3+) shows the localization of specific immune cells within the tumor and its surroundings in female animal (for male, see Supplementary Fig. 8); a dashed line marks the tumor edge; scale, 100 μm; the staining was performed for three animals, four sections each, a representative image is shown.