Skip to main content
. 2021 Feb 19;12(2):200. doi: 10.1038/s41419-021-03488-z

Fig. 2. Activation of TGFβ1 delays c-Myc induced hepatocarcinogenesis in mice.

Fig. 2

a Study design. FVB/N mice were injected with c-Myc/pT3/SB (N = 6) or c-Myc/ TGFβ1/SB (N = 9) plasmids. Mice were monitored and sacrificed when moribund. b Representative images of tumor and H&E staining in c-Myc/pT3 and c-Myc/TGFβ1 mouse livers at 5 weeks post injection (5 W p.i.). Scale bars: 200μm for 100X. c Representative images of Ki67 staining in c-Myc/ pT3 and c-Myc/TGFβ1 mouse livers at 5 weeks post injection (5 W p.i.). Red arrows indicate Ki67+ cells in c-Myc/TGFβ1 group. Scale bars: 100 μm for 200X. d Quantification of Ki67+ cells percentages in in c-Myc/pT3 and c-Myc/TGFβ1 mouse livers. Student t test was applied for statistical analysis. e Survival curve showing that TGFβ1 impaired c-Myc induced hepatocarcinogenesis in mice. Kaplan–Meier method and log-rank test were applied. P < 0.0001. f Representative images of liver, H&E, Ki67, and Cleaved Caspase-3 (C-C3) immunohistochemical staining in c-Myc/TGFβ1 group at different time points after injection. Green arrow indicates a tumor nodule on the surface of the right lobe of mouse liver. Red arrows indicate Ki67+ cells. Scale bars: 200 μm for H&E, 100 μm for Ki67 and C-C3.