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. 2021 Feb 19;12(2):200. doi: 10.1038/s41419-021-03488-z

Fig. 4. TGFβ1 activation promotes cell migration and invasion in c-Myc murine tumor derived HCC cell lines.

Fig. 4

a Western blotting results showing activation of Smad2/3 (p-Smad2/3) in TGFβ1 overexpressing HCC3-4 and HCC4-4 cells. Gapdh was used as a loading control. b, c Representative images and quantifications of cell healing assay at 0-hour (0 h) and after 48 hours (48 h) treatment for inducing TGFβ1 (N = 3) or EGFP (N = 3) expression in b HCC3-4 and c HCC4-4 cell lines. d, e Representative images and quantification of transwell migration assay 48 h after treatment for inducing TGFβ1 (N = 3) or EGFP (N = 3) expression in (d) HCC3-4 and (e) HCC4-4 cell lines. Student t test was applied for statistical analysis. P values were as shown. Experiments were conducted three times.