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. 2020 Aug 20;58(7):484–494. doi: 10.1136/jmedgenet-2020-106987

Figure 3.

Figure 3

Wildtype CIC regulation of FOLR1, PCFT and RFC1. (A) CIC DNA binding motifs distribution on FOLR1, PCFT and RFC1 gene. FOLR1 has two CIC binding motifs in promoter region, while PCF and RFC1 have only one CIC binding motif separately. (B) CIC overexpression by transfecting GFP-CIC plasmid and FOLR1, PCFT and RFC1 gene expression detected by qRT-PCR. CIC overexpression significantly increased FOLR1 and PCFT gene expression. (C) Luciferase assay of FOLR1 promoter indicated that wildtype CIC could stimulate FOLR1 promoter-luciferase expression, while mutant CIC had less ability in stimulating FOLR1 promoter-luciferase expression. Student's t-test was performed (*p<0.05; **p<0.005). (D) CIC knockdown by anti-CIC siRNA (si-CIC) and the regulation of three folate transport genes, FOLR1, PCFT and RFC1. Student's t-test was performed (*p<0.05). (E) CIC knockdown significantly decreased FOLR1 expression. Student's t-test was performed (*p<0.05).